A direct sandwich enzyme-linked immunosorbent assay for human serum apolipoprotein A-I

Cited 1 time in scopus
Metadata Downloads
Title
A direct sandwich enzyme-linked immunosorbent assay for human serum apolipoprotein A-I
Author(s)
Nam-Wook Jeong; Byeong-Cheol Jeon; Myung-Hoon Lee; Ju Won Kwak; Jin Q Kim; Soo-Kyung Kim; Young-Do Kim
Bibliographic Citation
Experimental and Molecular Medicine, vol. 28, no. 1, pp. 41-46
Publication Year
1996
Abstract
Apolipoprotein (apo) A-I, an abundant protein in high-density lipoproteins (HDL), has received considerable clinical attention as a negative predictor of coronary artery disease, because the plasma concentration of apo A-I has been shown to be inversely correlated with the incidence of coronary artery disease. A direct sandwich enzyme-linked immunosorbent assay (ELISA) for determination of the level of human serum apo A-I was developed using highly purified anti-apo A-I polyclonal antibodies raised in rabbits as the capturing antibody, and as the detecting antibody as well. Microtiter plates were coated with purified anti-apo A-I polyclonal antibodies. The concentration of apo A-I in samples was determined from the peroxidase color reaction. The sensitivity of the assay was determined to be 10 ng of apo A-I per ml of serum. The working range, where the standard curve showed a linearity, was between 10 and 100 ng/ml. The mean intra-assay and inter-assay coefficients of variation (CV) were < 5% and < 7%, respectively. No cross reactivities with serum albumin, apo A-II, B-100, C-I, C-II and E were detected. For the evaluation of validity of the assay, 20 serum samples of domestic subjects were employed, and the serum apo A-I measurement by the sandwich ELISA of this report was compared with those of Beekman's and Kallestad's immunonephelometry. The results indicated that there is a good correlation between the data obtained by the present ELISA method and by the two commercially available immunonephelometric assay methods (r = 0.95 and 0.84, respectively).
Keyword
Coronary artery diseasePolyclonal antibodiesSandwich ELISAApolipoprotein A-I
ISSN
I000-0028
Publisher
Springer-Nature Pub Group
DOI
http://dx.doi.org/10.1038/emm.1996.7
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.