Flow cytometric analysis of antibody producing cells using double immunofluorescent staining

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dc.contributor.authorGyun-Min Lee-
dc.contributor.authorJae Hoon Kim-
dc.contributor.authorHee Gu Lee-
dc.date.accessioned2017-04-19T08:54:28Z-
dc.date.available2017-04-19T08:54:28Z-
dc.date.issued1996-
dc.identifier.issn0951-208X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4031-
dc.description.abstractDouble fluorescent labeling, with fluorescein isothiocyanate (FITC)- labeled F(ab')2 specific for the heavy chain and R-phycoerythrin (R-PE)- labeled F(ab')2 specific for the light chain, was demonstrated as a convenient means for the accurate evaluation of a heterogeneous non- antibody-producing population. Furthermore, it could be used for monitoring the changes in each immunoglobulin (Ig) chain content of the cells during the batch culture, which will facilitate the study on antibody synthesis, assembly and secretion.-
dc.publisherSpringerko
dc.titleFlow cytometric analysis of antibody producing cells using double immunofluorescent staining-
dc.title.alternativeFlow cytometric analysis of antibody producing cells using double immunofluorescent staining-
dc.typeArticle-
dc.citation.titleBiotechnology Techniques-
dc.citation.number8-
dc.citation.endPage620-
dc.citation.startPage615-
dc.citation.volume10-
dc.contributor.affiliatedAuthorJae Hoon Kim-
dc.contributor.affiliatedAuthorHee Gu Lee-
dc.contributor.alternativeName이균민-
dc.contributor.alternativeName김재훈-
dc.contributor.alternativeName이희구-
dc.identifier.bibliographicCitationBiotechnology Techniques, vol. 10, no. 8, pp. 615-620-
dc.identifier.doi10.1007/BF00157372-
dc.description.journalClassN-
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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