Na+-dependent NADH : quinone oxidoreductase in the respiratory chain of the marine bacterium Marinomonas vaga

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dc.contributor.authorYoung Jae Kim-
dc.contributor.authorYong Ha Park-
dc.description.abstractThe Gram-negative marine bacterium Marinomonas vaga, which requires 0.5 M NaCl concentration for optimal growth, is slightly halophilic. The growth of M. vaga was highly resistant to the proton conductor, carbonyl cyanide m-chlorophenylhydrazone (CCCP) under alkaline pH conditions (pH 8.5) but very sensitive to CCCP under acidic pH conditions (pH 6.5). These results suggest that the respiratory chain-linked NADH oxidase system of M. vaga may lead to generation of a Na+ electrochemical gradient. In order to examine the existence of Na+-stimulated NADH oxidase in M. vaga, membrane fractions were prepared by the osmotic lysis method. The membrane-bound NADH oxidase oxidized both NADH and deamino-NADH as substrates and required Na+ for maximum activity. The maximum activity of NADH oxidase was obtained at about pH 8.5 in the presence of 0.2 M NaCl. The site of Na+-dependent activation in the NADH oxidase system was at the NADH:quinone oxidoreductase segment. The NADH oxidase and NADH:quinone oxidoreductase were very sensitive to the respiratory chain inhibitor, 2-heptyl-4-hydroxyquinoline-N-oxide (HQNO) in the presence of 0.2 M NaCl but highly resistant to another respiratory inhibitor, rotenone. Based on these findings, we conclude that M. vaga possesses the Na+-dependent NADH:quinone oxidoreductase that may function as an electrogenic Na+ pump.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleNa+-dependent NADH : quinone oxidoreductase in the respiratory chain of the marine bacterium Marinomonas vaga-
dc.title.alternativeNa+-dependent NADH : quinone oxidoreductase in the respiratory chain of the marine bacterium Marinomonas vaga-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.contributor.affiliatedAuthorYong Ha Park-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 6, no. 6, pp. 391-396-
dc.subject.keywordHalophilic marine bacteria-
dc.subject.keywordMarinomonas vaga-
dc.subject.keywordNa+ pump-
dc.subject.keywordNa+-dependent NADH-
dc.subject.keywordquinone oxidoreductase-
dc.subject.keywordRespiratory chain-
dc.subject.localHalophilic marine bacteria-
dc.subject.localMarinomonas vaga-
dc.subject.localNa+ pump-
dc.subject.localNa+-dependent NADH-
dc.subject.localquinone oxidoreductase-
dc.subject.localRespiratory chain-
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