Overexpression and simple purification of a truncated, immunologically reactive GST-HCV core (1-123) fusion protein

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Title
Overexpression and simple purification of a truncated, immunologically reactive GST-HCV core (1-123) fusion protein
Author(s)
Young Rim Seong; Eun Kyung Lee; See Young Choi; Seung Ki Chon; Dong Soo Im
Bibliographic Citation
Journal of Virological Methods, vol. 59, no. 1, pp. 13-21
Publication Year
1996
Abstract
A full-length and a truncated gene for the core protein of hepatitis C virus (HCV) were linked to the gene for glutathione S-transferase (GST), and the expression of each GST-HCV core fusion protein was analyzed. The truncated GST-HCV core (1-123) fusion protein was expressed as a mostly soluble and partly insoluble form comprising more than 50% of the total protein in Escherichia coli after induction by isopropylthio-β-D-galactoside (IPTG), while the full length GST-HCV core (1-191) fusion protein was not expressed, suggesting that the hydrophobic carboxy terminal region in the core protein affects its expression. In addition, the GST-HCV core (1-123) fusion protein purified by GST-agarose chromatography reacted specifically with an anti-HCV serum from a patient.
Keyword
Core proteinHepatitis C virusPurification
ISSN
0166-0934
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0166-0934(95)01995-2
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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