EcoRI variant N199H has enhanced specific activity
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Jae Jong Kim | - |
| dc.contributor.author | Kyung Tai Min | - |
| dc.contributor.author | Myung Hee Kim | - |
| dc.contributor.author | S J Augh | - |
| dc.contributor.author | Byung-Dong Kim | - |
| dc.contributor.author | Dae Sil Lee | - |
| dc.date.accessioned | 2017-04-19T08:54:32Z | - |
| dc.date.available | 2017-04-19T08:54:32Z | - |
| dc.date.issued | 1996 | - |
| dc.identifier.issn | 0378-1119 | - |
| dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4066 | - |
| dc.description.abstract | The Asn199 residue of the EcoRI restriction endonuclease has been replaced with other amino acids to investigate whether it mediates nucleotide recognition or catalytic activity. Cassette mutagenesis gave variants of EcoRI: N199D, N199H, N199L, N199R, N199S and N199V. Their relative cleavage rates were found to be in the following order: N199H > EcoRI (wild type; wt) > N199L > N199V > N199S > N199R > N199D. In particular, EcoRI variant N199H showed about a twofold higher specific activity than that of the wt enzyme. | - |
| dc.publisher | Elsevier | - |
| dc.title | EcoRI variant N199H has enhanced specific activity | - |
| dc.title.alternative | EcoRI variant N199H has enhanced specific activity | - |
| dc.type | Article | - |
| dc.citation.title | Gene | - |
| dc.citation.number | 0 | - |
| dc.citation.endPage | 130 | - |
| dc.citation.startPage | 129 | - |
| dc.citation.volume | 171 | - |
| dc.contributor.affiliatedAuthor | Myung Hee Kim | - |
| dc.contributor.affiliatedAuthor | Dae Sil Lee | - |
| dc.contributor.alternativeName | 김재종 | - |
| dc.contributor.alternativeName | 민경태 | - |
| dc.contributor.alternativeName | 김명희 | - |
| dc.contributor.alternativeName | Augh | - |
| dc.contributor.alternativeName | 김병동 | - |
| dc.contributor.alternativeName | 이대실 | - |
| dc.identifier.bibliographicCitation | Gene, vol. 171, pp. 129-130 | - |
| dc.identifier.doi | 10.1016/0378-1119(96)00003-0 | - |
| dc.subject.keyword | Site-directed mutagenesis | - |
| dc.subject.local | Site-directed mutagenesis | - |
| dc.subject.local | site-directed mutagenesis | - |
| dc.description.journalClass | Y | - |
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