Characterization of PCP-degrading bacteria isolated from PCP-contaminated soils = 염소화페놀 오염토양에서 분리한 Pentachlorophenol 분해균주의 특성

Abstract

For the purpose of development of bioremediation technology for soil contamonated by chlorinated phenols, this study was focused on the isolation and characterization of bacteria capable of degrading chlorinated phenols, the establishment of analytical methods for chlorinated phenols, and the investigation of the contaminated sites. One site near the Incheon Industrial Complex was identified as a pentachlorophenol (PCP)-contaminated spot. The soil brought from the PCP-contaminated site contained 50-100μg/g wet soil of PCP. Many bacterial strains capable of growing on a minimal medium containing PCP were isolated from 15 soil samples collected throught the land, and among them, 10 active isolated were finally selected for the further studies on the biodegradability and for the use in in situ bioremediation of contaminated soil. These isolated showed species-specific pattern in PCP-decrease and cell growth in a minimal medium containing 500-1,000mg/l PCP. Strain bu1 degraded 90% of PCP at 216 hrs after incubation. Expecially, strain Bu34 was capable of degrading 4,000ㅡmg/l PCP and was identified as Pseudomonas putida Bu34. It is seemed that the isolated active bacteria could be effectively used for the bioremediation of PCP-contaminated site.