Immunofluorescent quantification of tyrosine phosphorylated proteins by flow cytometric analysis

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dc.contributor.authorDo Young Yoon-
dc.contributor.authorSun Young Yoon-
dc.contributor.authorWoo Suk Koh-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorTai Wha Chung-
dc.contributor.authorMi Young Han-
dc.date.accessioned2017-04-19T08:54:35Z-
dc.date.available2017-04-19T08:54:35Z-
dc.date.issued1997-
dc.identifier.issn0951-208X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4088-
dc.description.abstractTo measure the quantity of tyrosine phosphorylated proteins in cells, we have used a flow cytometry technique with fluorescein isothiocyanate-labeled anti-phosphotyrosine antibody (FITC-αPY mAb). The analysis was applied to the phosphotyrosine titration and showed an optimum amount of FITC-αPY mAb (30 μg/1 x 106cells). The staining specificity of our assay was tested by the addition of exogenous competitors, showing a specific inhibition by phosphotyrosine but not by phosphoserine, phosphothreonine, or tyrosine. The assay was also able to elucidate the inhibitory effect on tyrosine phosphorylation of genistein, a protein tyrosine kinase inhibitor. These results imply that immunofluorescent quantification assay using a flow cytometer could be a useful technique to determine the intracellular level of tyrosine phosphorylated protein.-
dc.publisherSpringerko
dc.titleImmunofluorescent quantification of tyrosine phosphorylated proteins by flow cytometric analysis-
dc.title.alternativeImmunofluorescent quantification of tyrosine phosphorylated proteins by flow cytometric analysis-
dc.typeArticle-
dc.citation.titleBiotechnology Techniques-
dc.citation.number3-
dc.citation.endPage211-
dc.citation.startPage209-
dc.citation.volume11-
dc.contributor.affiliatedAuthorDo Young Yoon-
dc.contributor.affiliatedAuthorSun Young Yoon-
dc.contributor.affiliatedAuthorWoo Suk Koh-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.affiliatedAuthorMi Young Han-
dc.contributor.alternativeName윤도영-
dc.contributor.alternativeName윤선영-
dc.contributor.alternativeName고우석-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName정태화-
dc.contributor.alternativeName한미영-
dc.identifier.bibliographicCitationBiotechnology Techniques, vol. 11, no. 3, pp. 209-211-
dc.description.journalClassN-
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