DC Field | Value | Language |
---|---|---|
dc.contributor.author | Do Young Yoon | - |
dc.contributor.author | Sun Young Yoon | - |
dc.contributor.author | Woo Suk Koh | - |
dc.contributor.author | In Seong Choe | - |
dc.contributor.author | Tai Wha Chung | - |
dc.contributor.author | Mi Young Han | - |
dc.date.accessioned | 2017-04-19T08:54:35Z | - |
dc.date.available | 2017-04-19T08:54:35Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0951-208X | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4088 | - |
dc.description.abstract | To measure the quantity of tyrosine phosphorylated proteins in cells, we have used a flow cytometry technique with fluorescein isothiocyanate-labeled anti-phosphotyrosine antibody (FITC-αPY mAb). The analysis was applied to the phosphotyrosine titration and showed an optimum amount of FITC-αPY mAb (30 μg/1 x 106cells). The staining specificity of our assay was tested by the addition of exogenous competitors, showing a specific inhibition by phosphotyrosine but not by phosphoserine, phosphothreonine, or tyrosine. The assay was also able to elucidate the inhibitory effect on tyrosine phosphorylation of genistein, a protein tyrosine kinase inhibitor. These results imply that immunofluorescent quantification assay using a flow cytometer could be a useful technique to determine the intracellular level of tyrosine phosphorylated protein. | - |
dc.publisher | Springer | ko |
dc.title | Immunofluorescent quantification of tyrosine phosphorylated proteins by flow cytometric analysis | - |
dc.title.alternative | Immunofluorescent quantification of tyrosine phosphorylated proteins by flow cytometric analysis | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Techniques | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 211 | - |
dc.citation.startPage | 209 | - |
dc.citation.volume | 11 | - |
dc.contributor.affiliatedAuthor | Do Young Yoon | - |
dc.contributor.affiliatedAuthor | Sun Young Yoon | - |
dc.contributor.affiliatedAuthor | Woo Suk Koh | - |
dc.contributor.affiliatedAuthor | In Seong Choe | - |
dc.contributor.affiliatedAuthor | Tai Wha Chung | - |
dc.contributor.affiliatedAuthor | Mi Young Han | - |
dc.contributor.alternativeName | 윤도영 | - |
dc.contributor.alternativeName | 윤선영 | - |
dc.contributor.alternativeName | 고우석 | - |
dc.contributor.alternativeName | 최인성 | - |
dc.contributor.alternativeName | 정태화 | - |
dc.contributor.alternativeName | 한미영 | - |
dc.identifier.bibliographicCitation | Biotechnology Techniques, vol. 11, no. 3, pp. 209-211 | - |
dc.description.journalClass | N | - |
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