Differential expression of ferritin heavy chain in THP-1 cells infected with Mycobacterium bovis BCG

Cited 4 time in scopus
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Title
Differential expression of ferritin heavy chain in THP-1 cells infected with Mycobacterium bovis BCG
Author(s)
Jong-Seok Lim; Seung-Hwan Lee; Eunsik Lee; Young Kang; Jae Wha Kim; Jin Koo Kim; Hyeon Hoe Kim; Chongwook Lee; Sang Jae Kim; Gill Han Bai; Hee Gu Lee; Kwang Dong Kim; Tai Wha Chung; Yong Kyung Choe
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 43, no. 5, pp. 981-988
Publication Year
1997
Abstract
To identify the host genes induced or suppressed by infection of mycobacteria, the reverse transcriptase polymerase chain reaction (RT-PCR) and the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) methods were used. In this study, cDNAs complement to mRNA extracted from human peripheral monocyte derived naive THP-1 cells, THP-1 cells infected with live Mycobacterium bovis BCG, THP-1 cells treated with heat-killed BCG, and THP-I cells incubated with IgG-coated glass-beads were compared on the sequencing gel. One (TG2-1) of the clones selected by DD RT-PCR is 446 bp long and is identical to human ferritin heavy (H) chain gene. Northern blot analysis confirmed that ferritin H chain gene has been markedly over-expressed in monocytic THP-1 cells incubated with live and dead M. bovis BCG. Differential display techniques of host genes whose expression levels were varied by infection of mycobacteria could provide information about the response of macrophages to mycobacterial infection.
Keyword
BCGDifferential displayFerritinTHP-1
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549700204791
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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