Molecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis

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dc.contributor.authorMi Young Han-
dc.contributor.authorMi Young Son-
dc.contributor.authorSeung-Hwan Lee-
dc.contributor.authorJin Koo Kim-
dc.contributor.authorJae Suk Huh-
dc.contributor.authorJeong Hyun Kim-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorTai Wha Chung-
dc.contributor.authorYong Kyung Choe-
dc.date.accessioned2017-04-19T08:54:36Z-
dc.date.available2017-04-19T08:54:36Z-
dc.date.issued1997-
dc.identifier.issn1039-9712-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4098-
dc.description.abstractA gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1011 bp encoding isopropylmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp.-
dc.publisherWiley-
dc.titleMolecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis-
dc.title.alternativeMolecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis-
dc.typeArticle-
dc.citation.titleBiochemistry and Molecular Biology International-
dc.citation.number4-
dc.citation.endPage663-
dc.citation.startPage657-
dc.citation.volume41-
dc.contributor.affiliatedAuthorMi Young Han-
dc.contributor.affiliatedAuthorSeung-Hwan Lee-
dc.contributor.affiliatedAuthorJin Koo Kim-
dc.contributor.affiliatedAuthorJeong Hyun Kim-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.affiliatedAuthorYong Kyung Choe-
dc.contributor.alternativeName한미영-
dc.contributor.alternativeName손미영-
dc.contributor.alternativeName이승환-
dc.contributor.alternativeName김진구-
dc.contributor.alternativeName허재숙-
dc.contributor.alternativeName김정현-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName정태화-
dc.contributor.alternativeName최용경-
dc.identifier.bibliographicCitationBiochemistry and Molecular Biology International, vol. 41, no. 4, pp. 657-663-
dc.identifier.doi10.1080/15216549700201701-
dc.subject.keywordBCG-
dc.subject.keywordIsopropylmalate dehydrogenase gene-
dc.subject.keywordleuB-
dc.subject.keywordM. tuberculosis-
dc.subject.localBCG-
dc.subject.localIsopropylmalate dehydrogenase gene-
dc.subject.localleuB-
dc.subject.localM. tuberculosis-
dc.description.journalClassY-
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