DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mi Young Han | - |
dc.contributor.author | Mi Young Son | - |
dc.contributor.author | Seung-Hwan Lee | - |
dc.contributor.author | Jin Koo Kim | - |
dc.contributor.author | Jae Suk Huh | - |
dc.contributor.author | Jeong Hyun Kim | - |
dc.contributor.author | In Seong Choe | - |
dc.contributor.author | Tai Wha Chung | - |
dc.contributor.author | Yong Kyung Choe | - |
dc.date.accessioned | 2017-04-19T08:54:36Z | - |
dc.date.available | 2017-04-19T08:54:36Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 1039-9712 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4098 | - |
dc.description.abstract | A gene responsible for the biosynthesis of leucine has been cloned by the complementation of the Escherichia coli leuB6 auxotroph mutant after transformation with the Mycobacterium bovis BCG genomic DNA library, which was constructed by ligating the partially digested BCG DNA with Sau3AI into the pUC19 digested with BamHI. Sequencing of the leuB gene of BCG revealed an ORF (open reading frame) of 1011 bp encoding isopropylmalate dehydrogenase with a calculated molecular weight of 42 kDa. The leuB gene of Mycobacterium tuberculosis isolated from Korean tuberculosis patient is shown to be identical to that of BCG except one bp. | - |
dc.publisher | Wiley | - |
dc.title | Molecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis | - |
dc.title.alternative | Molecular cloning of the LEUB genes from Mycobacterium bovis BCG and Mycobacterium tuberculosis | - |
dc.type | Article | - |
dc.citation.title | Biochemistry and Molecular Biology International | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 663 | - |
dc.citation.startPage | 657 | - |
dc.citation.volume | 41 | - |
dc.contributor.affiliatedAuthor | Mi Young Han | - |
dc.contributor.affiliatedAuthor | Seung-Hwan Lee | - |
dc.contributor.affiliatedAuthor | Jin Koo Kim | - |
dc.contributor.affiliatedAuthor | Jeong Hyun Kim | - |
dc.contributor.affiliatedAuthor | In Seong Choe | - |
dc.contributor.affiliatedAuthor | Tai Wha Chung | - |
dc.contributor.affiliatedAuthor | Yong Kyung Choe | - |
dc.contributor.alternativeName | 한미영 | - |
dc.contributor.alternativeName | 손미영 | - |
dc.contributor.alternativeName | 이승환 | - |
dc.contributor.alternativeName | 김진구 | - |
dc.contributor.alternativeName | 허재숙 | - |
dc.contributor.alternativeName | 김정현 | - |
dc.contributor.alternativeName | 최인성 | - |
dc.contributor.alternativeName | 정태화 | - |
dc.contributor.alternativeName | 최용경 | - |
dc.identifier.bibliographicCitation | Biochemistry and Molecular Biology International, vol. 41, no. 4, pp. 657-663 | - |
dc.identifier.doi | 10.1080/15216549700201701 | - |
dc.subject.keyword | BCG | - |
dc.subject.keyword | Isopropylmalate dehydrogenase gene | - |
dc.subject.keyword | leuB | - |
dc.subject.keyword | M. tuberculosis | - |
dc.subject.local | BCG | - |
dc.subject.local | Isopropylmalate dehydrogenase gene | - |
dc.subject.local | leuB | - |
dc.subject.local | M. tuberculosis | - |
dc.description.journalClass | Y | - |
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