Determination of isoprenyl and lavandulyl positions of flavonoids from Sophora flavescens by NMR experiment

Abstract

All fifteen flavonoids (1-15) have been isolated from the roots of Sophora flavescens (Leguminosae) as active principles of the cytotoxic property toward human tumor cell lines such as A549, SK-OV-3, SK-Mel-2, XF498 and HCT15, in vitro. By means of spectral analyses, particularly by the aid of various two dimensional NMR experiments, all 1H-NMR and 13C-NMR signals of 1-15 were completely assigned, and thus the structures of 1-15 were established unambiguously.