Cloning and phylogenetic analysis of chitin synthase gene from entomopathogenic fungus, Beauveria brongniartii

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Title
Cloning and phylogenetic analysis of chitin synthase gene from entomopathogenic fungus, Beauveria brongniartii
Author(s)
Jin Sik Nam; Dong-Hun Lee; Ho Yong Park; Kyung Sook Bae
Bibliographic Citation
Journal of Microbiology, vol. 35, no. 3, pp. 222-227
Publication Year
1997
Abstract
DNA fragments homologous to chitin synthase gene were amplified from the genomic DNA of Beauveria brongniartii by PCR using degenerate primers. Cloning and sequencing of the PCR-amplified fragments led to the identification of a gene, designated BbCHS1. Comparison of the deduced amino acid sequence of BbCHS1 with those of other Euascomycetes revealed that BbCHS1 is a gene for class II chitin synthase. The BlastP search of the deduced amino acid sequence of BbCHS1 displayed the highest rate of similarity, 95.8%, with CHS2 of Metarhizium anisopliae. Phylogenetic analysis of the amino acid sequences confirmed the taxonomic and evolutionary position of B. brongniartii, which was previously derived by traditional fungal classification based on morphological features.
Keyword
Beauveria brongniartiichitin synthase genePCRphylogeny
ISSN
1225-8873
Publisher
Microbiological Society Korea
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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