Production of cyclodextrin from starch by cyclodextrin glycosyltransferase from Bacillus firmus and characterization of purified enzyme

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Title
Production of cyclodextrin from starch by cyclodextrin glycosyltransferase from Bacillus firmus and characterization of purified enzyme
Author(s)
Dong Gu Yim; H H Sato; Yong Ha Park; Y K Park
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 18, pp. 402-405
Publication Year
1997
Abstract
During screening for cyclodextrin-forming microorganisms, an alkalophilic Bacillus sp, which produced high activity of cyclodextrin glycosyltransferase, was isolated and identified as Bacillus firmus. The crude enzyme transformed starch to mainly β- and γ-cyclodextrin. The purified enzyme had an optimum pH of 7.5-8.5 and its optimum temperature was 65°C, which is the highest optimum temperature as compared to other cyclodextrin glycosyltransferases except that produced by Bacillus amyloliquefaciens.
Keyword
cyclodextrincyclodextrin glycosyltransferasestarchalkalophilic bacillusBacillus firmus
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1038/sj.jim.2900400
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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