Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains

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dc.contributor.authorJung Hoon Yoon-
dc.contributor.authorSung-Taik Lee-
dc.contributor.authorSam-Bong Kim-
dc.contributor.authorWon-Yong Kim-
dc.contributor.authorM Goodfellow-
dc.contributor.authorYong Ha Park-
dc.date.accessioned2017-04-19T08:54:40Z-
dc.date.available2017-04-19T08:54:40Z-
dc.date.issued1997-
dc.identifier.issn0020-7713-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4130-
dc.description.abstractTwenty-one strains of Saccharomonospora azurea, Saccharomonospora cyanea, Saccharomonospora glauca, Saccharomonospora viridis, and 'Saccharomonospora caesia' were examined to evaluate the discriminatory value of 16S ribosomal DNA (rDNA) fingerprints. The 16S rDNAs were amplified by PCR by using oligonucleotide primers complementary to 16S rRNA genes. A restriction fragment length polymorphism (RFLP) analysis of the 16S rDNAs was performed with Smal and MluI. The four validly described Saccharomonospora species could be differentiated on the basis of their characteristic 16S rDNA restriction patterns. The strains of 'S. caesia' gave a restriction pattern identical to that of S. azurea K161(T) (T = type strain). This result was anticipated from the previous report that S. azurea K161(T) and the strains of 'S. caesia' have identical 16S rRNA sequences. We found that purification of amplified 16S rDNA products following PCR was necessary for our RFLP analysis.-
dc.publisherMicrobiology Soc-
dc.titleRestriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains-
dc.title.alternativeRestriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains-
dc.typeArticle-
dc.citation.titleInternational Journal of Systematic and Evolutionary Microbiology-
dc.citation.number1-
dc.citation.endPage114-
dc.citation.startPage111-
dc.citation.volume47-
dc.contributor.affiliatedAuthorJung Hoon Yoon-
dc.contributor.affiliatedAuthorSam-Bong Kim-
dc.contributor.affiliatedAuthorWon-Yong Kim-
dc.contributor.affiliatedAuthorYong Ha Park-
dc.contributor.alternativeName윤정훈-
dc.contributor.alternativeName이성택-
dc.contributor.alternativeName김삼봉-
dc.contributor.alternativeName김원용-
dc.contributor.alternativeNameGoodfellow-
dc.contributor.alternativeName박용하-
dc.identifier.bibliographicCitationInternational Journal of Systematic and Evolutionary Microbiology, vol. 47, no. 1, pp. 111-114-
dc.identifier.doi10.1099/00207713-47-1-111-
dc.description.journalClassY-
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