Enhanced In vitro refolding selectivity of the recombinant human insulin-like growth factor I

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSun-Ok Kim-
dc.contributor.authorYoung Ik Lee-
dc.date.accessioned2017-04-19T08:54:41Z-
dc.date.available2017-04-19T08:54:41Z-
dc.date.issued1997-
dc.identifier.issn0951-208X-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4137-
dc.description.abstractA systematic study was carried out to optimize production of biologically active recombinant IGF-I with native conformation. Careful optimization of buffer carbonate, pH (9.5), protein concentration (0.5 mg/ml), temperature (25°C), and disulfide-exchange reagents (2 mM reduced glutathione/1 mM oxidized glutathione, 1 mM cysteine, 1 mM β-mercaptoethanol, and 2 mM dithiothreitol) allowed a yield of correctly folded recombinant IGF-I as high as 80%, which may be useful for large scale production of IGF-I.-
dc.publisherSpringerko
dc.titleEnhanced In vitro refolding selectivity of the recombinant human insulin-like growth factor I-
dc.title.alternativeEnhanced In vitro refolding selectivity of the recombinant human insulin-like growth factor I-
dc.typeArticle-
dc.citation.titleBiotechnology Techniques-
dc.citation.number2-
dc.citation.endPage89-
dc.citation.startPage85-
dc.citation.volume11-
dc.contributor.affiliatedAuthorSun-Ok Kim-
dc.contributor.affiliatedAuthorYoung Ik Lee-
dc.contributor.alternativeName김선옥-
dc.contributor.alternativeName이영익-
dc.identifier.bibliographicCitationBiotechnology Techniques, vol. 11, no. 2, pp. 85-89-
dc.subject.keywordGrowth Factor-
dc.subject.keywordGlutathione-
dc.subject.keywordCysteine-
dc.subject.keywordProtein Concentration-
dc.subject.keywordSystematic Study-
dc.subject.localgrowth factor-
dc.subject.localGrowth Factor-
dc.subject.localGrowth factors-
dc.subject.localGrowth factor-
dc.subject.localglutathione-
dc.subject.localGlutathione-
dc.subject.localCysteine-
dc.subject.localcysteine-
dc.subject.localProtein Concentration-
dc.subject.localSystematic Study-
dc.description.journalClassN-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.