DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sun-Ok Kim | - |
dc.contributor.author | Young Ik Lee | - |
dc.date.accessioned | 2017-04-19T08:54:41Z | - |
dc.date.available | 2017-04-19T08:54:41Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0951-208X | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4137 | - |
dc.description.abstract | A systematic study was carried out to optimize production of biologically active recombinant IGF-I with native conformation. Careful optimization of buffer carbonate, pH (9.5), protein concentration (0.5 mg/ml), temperature (25°C), and disulfide-exchange reagents (2 mM reduced glutathione/1 mM oxidized glutathione, 1 mM cysteine, 1 mM β-mercaptoethanol, and 2 mM dithiothreitol) allowed a yield of correctly folded recombinant IGF-I as high as 80%, which may be useful for large scale production of IGF-I. | - |
dc.publisher | Springer | ko |
dc.title | Enhanced In vitro refolding selectivity of the recombinant human insulin-like growth factor I | - |
dc.title.alternative | Enhanced In vitro refolding selectivity of the recombinant human insulin-like growth factor I | - |
dc.type | Article | - |
dc.citation.title | Biotechnology Techniques | - |
dc.citation.number | 2 | - |
dc.citation.endPage | 89 | - |
dc.citation.startPage | 85 | - |
dc.citation.volume | 11 | - |
dc.contributor.affiliatedAuthor | Sun-Ok Kim | - |
dc.contributor.affiliatedAuthor | Young Ik Lee | - |
dc.contributor.alternativeName | 김선옥 | - |
dc.contributor.alternativeName | 이영익 | - |
dc.identifier.bibliographicCitation | Biotechnology Techniques, vol. 11, no. 2, pp. 85-89 | - |
dc.subject.keyword | Growth Factor | - |
dc.subject.keyword | Glutathione | - |
dc.subject.keyword | Cysteine | - |
dc.subject.keyword | Protein Concentration | - |
dc.subject.keyword | Systematic Study | - |
dc.subject.local | growth factor | - |
dc.subject.local | Growth Factor | - |
dc.subject.local | Growth factors | - |
dc.subject.local | Growth factor | - |
dc.subject.local | glutathione | - |
dc.subject.local | Glutathione | - |
dc.subject.local | Cysteine | - |
dc.subject.local | cysteine | - |
dc.subject.local | Protein Concentration | - |
dc.subject.local | Systematic Study | - |
dc.description.journalClass | N | - |
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