Molecular cloning and expression of cDNAs encoding mouse Galβ1,3(4)GlcNAc α2,3-sialyltransferase(mST3Gal III) and Galβ1,4(3)GlcNAc α2,3-sialyltransferase(mST3Gal Ⅳ)

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Title
Molecular cloning and expression of cDNAs encoding mouse Galβ1,3(4)GlcNAc α2,3-sialyltransferase(mST3Gal III) and Galβ1,4(3)GlcNAc α2,3-sialyltransferase(mST3Gal Ⅳ)
Author(s)
Kyoung-Sook Kim; Cheorl Ho Kim; Deug Yong Shin; Young Choon Lee
Bibliographic Citation
BMB Reports, vol. 30, no. 2, pp. 95-100
Publication Year
1997
Abstract
Two kinds of cDNA encoding mouse Galβ1,3(4)GlcNAc α2,3-sialyltransferase (mST3Gal III) and Galβ1,4(3)GlcNAc α2,3-sialyltransferase (mST3Gal IV) were isolated from mouse brain cDNA library by means of a PCR-based approach. The cDNA sequences included an open reading frame coding for proteins of 374 and 333 amino acids, respectively, and the primary structure of these enzymes suggested a putative domain structure consisting of four regions, like that in other glycosyltransferases. The deduced amino acid sequences of mST3Gal III and IV showed a 98% and 89% identity with rat ST3Gal III and human ST3Gal IV, respectively. Northern analysis indicated that the expression of mST3Gal III mRNA was abundant in heart, liver and adult brain, while that of mST3Gal IV mRNA was detected in all tissues tested except for testis, but the level was the highest in liver. Soluble forms of mST3Gal III and IV transiently expressed in COS cells exhibited enzyme activity toward acceptor substrates containing the terminal either Galβ1,3GlcNAc or Galβ1, 4GlcNAc sequences. The substrate preferences of both enzymes were stronger for tetrasaccharides than for disaccharides.
Keyword
cDNA cloningexpressionsialyltransferase
ISSN
1225-8687
Publisher
Korea Soc-Assoc-Inst
Type
Article
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1. Journal Articles > Journal Articles
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