Expression of cry1C gene using a segregationally stable shuttle vector in Bacillus thuringiensis = Bacillus thuringiensis 내에서 안정한 벡타를 이용한 cry1C 유전자의 발현

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Title
Expression of cry1C gene using a segregationally stable shuttle vector in Bacillus thuringiensis = Bacillus thuringiensis 내에서 안정한 벡타를 이용한 cry1C 유전자의 발현
Author(s)
Soo Keun Choi; Keun-Hee Oh; Jeong Il Kim; Seung Hwan Park
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 25, no. 6, pp. 566-570
Publication Year
1997
Abstract
During sporulation, Bacillus thuringiensis strains produce crystals consist of toxin proteins high- ly specific against insect pests. Their host specificities are desirable from a standpoint of environmental safety, but also limit market potential. Thus, development of improved Bacillus thuringiensis strains having broad host spectrum will contribute to increase its use. For the construction of Bacillus thuringiensis strain having broad host spectrum, we cloned cry1C gene encoding a toxin protein highly toxic against Spodoptera exigua from a B. thuringiensis isolate and constructed two recombinant plasmids, pUBC1C and p1C60. The plasmid pUBC1C has a replication origin of the natural plasmid pBC16 from B. cereus which is closely related species to B. thuringiensis, and the pBC16 was known to be replicated by rolling-circle mechanism. The plasmid p1C60 has a replication origin of a resident 60 MDa plasmid from B. thuringiensis subsp. kurstaki HD263, and it is believed that the p1C60 is replicated in a theta mode. The two plasmids were introduced into B. thuringiensis subsp. kurstaki cryB strain, and the transformed strains produced well-shaped bipyramidal crystals. We confirmed the expression of the cry1C gene by SDS-PAGE, and Western blotting. By investigating the segregational stability, it was found that the plasmid p1C60 is more stable than the pUBC1C.
Keyword
Bacillus thuringiensisCry1cExpressionSegregational stability
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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