Medium optimization for production of thermostable alkaline proteases from Bacillus licheniformis NS70 = Bacillus licheniformis NS70으로부터 내열성 alkaline protease 생산을 위한 배지최적화

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Title
Medium optimization for production of thermostable alkaline proteases from Bacillus licheniformis NS70 = Bacillus licheniformis NS70으로부터 내열성 alkaline protease 생산을 위한 배지최적화
Author(s)
Ja-Hyup Koo; In-Jae Choi; Hee-Sop Nam; Hyung-Jae Lee; Zae-Ik Shin; Tae Kwang Oh
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 25, no. 2, pp. 207-211
Publication Year
1997
Abstract
Media optimization for the production of thermostable protease specifically hydrolyzing defatted soybean meal (DSM) from Bacillus licheniformis NS70 was performed by two methods, one-at-a-time method and response surface methodology (RSM). The best carbon source and nitrogen source for the protease production were lactose and DSM, respectively. The maximum protease production estimated by RSM was 606 U/L at 1.11% lactose and 0.43% DSM, the value of which was nearly consistent to the experimental value of 599 U/L. Yeast extract suppressed the protease production. The medium pH was slightly increased at the beginning stage of fermentation, and it tended to decrease after 8 hours. The optimal pH for the protease production was 7.2 in the batch fermentation.
Keyword
Medium optimizationProtease productionResponse surface methodology
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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