Expression analysis of a bovine β-casein/human lactoferrin hybrid gene in transgenic mice

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Title
Expression analysis of a bovine β-casein/human lactoferrin hybrid gene in transgenic mice
Author(s)
Sun Jung Kim; Ko Woon Lee; Dae Yeul Yu; Yong Mahn Han; Chul Sang Lee; Myung Soo Nam; Hyung-Bae Moon; Kyung Kwang Lee
Bibliographic Citation
Journal of Reproduction and Development, vol. 43, no. 2, pp. 143-149
Publication Year
1997
Abstract
In our previous study, using a bovine β-casein/human lactoferrin hybrid gene, transgenic mice were generated to target the expression of human lactoferrin (hLF) into the mammary glands. Here, we report the fidelity of transgene expression by eleven transgenic mice and their progeny. Expression of hLF in lactating mice was demonstrated by northern and western blots, ELISA, and immunohistochemical staining. Northern blot analysis in five different tissues revealed that the transgene was expressed in the mammary gland. hLF was expressed in five of eight transgenic mouse lines analyzed by ELISA. The expression level of hLF in milk at day 10 of lactation ranged from 2.5 to 200 μg/ml. The apparent molecular weight of the recombinant hLF was indistinguishable from the native form upon immunoblotting. Immunohistochemical staining revealed that hLF in transgenic mice was exclusively expressed in the epithelial cells of mammary gland. The transgenic mice produced in this study provide a model for the eventual generation of transgenic farm animals producing high levels of hLF in their milk.
Keyword
Bovine β-caseinHuman lactoferrinMammary glandTransgenic mouse
ISSN
I000-0068
Publisher
Society Reproduction & Development-Srd
DOI
http://dx.doi.org/10.1262/jrd.43.143
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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