The use of multiple antigenic peptide (MAP) in the immunodiagnosis of human immunodeficiency virus infection

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Title
The use of multiple antigenic peptide (MAP) in the immunodiagnosis of human immunodeficiency virus infection
Author(s)
Song Yub Shin; Myung Kyu Lee; Sun Young Kim; So Youn Jang; Kyung Soo Hahm
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 43, no. 4, pp. 713-721
Publication Year
1997
Abstract
In order to develop an ELISA system for the antibody detection of HIV-1 or HIV-2 infections, MAPs for HIV-1 gp41(584-618) and HIV-2 gp36(574-602) corresponding to the immunodominant regions of HIV-1 gp41 and HIV-2 gp36 were used as coating antigens in the ELISA. The MAPs were synthesized by the solid phase method using Fmoc-Lys(Fmoc)-OH and their molecular weights were confirmed by tricine gel electrophoresis. The MAPs reacted with all HIV positive sera (64 samples), but did not react with HIV negative sera (48 samples) obtained from healthy blood donors. The MAPs showed high sensitivity and specificity in anti-HIV 1/2 combo panel and anti-HIV-1 seroconversion panels. The results indicated that the ELISA system using synthetic MAPs of gp41(584-618) and gp36(574-602) as coating antigens can be used as an analytical system for the immunodiagnosis of HIV-1 or HIV-2 infections.
Keyword
MAPimmunodiagnosisantibody detectionHIV-1 gp41(584-618)HIV-2 gp36(574-602)
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549700204521
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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