High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant

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Title
High production of D-β-hydroxyisobutyric acid from methacrylic acid by Candida rugosa and its mutant
Author(s)
In Young Lee; Chul Ho Kim; B K Yeon; W K Hong; Eui Sung Choi; Sang Ki Rhee; Young Hoon Park; D H Sung; W H Baek
Bibliographic Citation
Bioprocess Engineering, vol. 16, pp. 247-252
Publication Year
1997
Abstract
Production of D-β-hydroxyisobutyric acid (D-HIBA) from methacrylic acid (MA) was investigated using Candida rugosa IFO 0750 and its mutant. Cell growth decreased as the MA concentration increased and was inhibited at D-HIBA concentrations higher than 30 g/l. Optimal MA concentration for D-HIBA production was in the range of 10-20 g/l. It was also noted that cell growth and D-HIBA production were inhibited by higher concentrations of Na+, K+, and NH4+, which were required for pH control during cultivation. With a suitably designed feeding mode of MA, the parent strain produced 65 g/l of D-HIBA after 120 h of fed-batch cultivation, but molar conversion yield of D-HIBA was less than 40%. The mutant, unable to assimilate propionic acid, produced as high as 70 g/l of D-HIBA in the same culture period with a molar conversion yield of more than 70%.
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/PL00008944
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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