Identification of a gene cluster of biosynthetic genes of rubradirin substructures in S. achromogenes var. rubradiris NRRL3061

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dc.contributor.authorJae-Kyung Sohng-
dc.contributor.authorTae-Jin Oh-
dc.contributor.authorJung Joon Lee-
dc.contributor.authorChun-Gyu Kim-
dc.date.accessioned2017-04-19T08:54:51Z-
dc.date.available2017-04-19T08:54:51Z-
dc.date.issued1997-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4209-
dc.description.abstractRubradirin, an ansamycin antibiotic has been purified from Streptomyces achromogenes var. rubradiris NRRL3061. It consists of four distinct structural moieties, rubransarol, 3-amino-4-hydroxy-coumarin, dihydroxydipicolinic acid, and 2,6-dideoxynitrosugar (DNS). Polymerase chain reaction (PCR) primers were designed based on consensus sequences of dTDP-D-glucose 4,6-dehydratase, one of enzymes involved in the biosynthesis of 2,6-dideoxysugar. A PCR product was obtained from S. achromogenes var. rubradiris. Hybridization of the PCR product to a cosmid library constructed from S. achromogenes genomic DNA has led to the identification of three unlinked regions of DNA. One of three kinds of cosmid clones contains homologues of dTDP-D-glucose 4,6-dehydratase, 3-amino-5-hydroxybenzoic acid (AHBA) synthase, and eryA genes. The size of the gene homologous to eryA is 30 kb, and the AHBA synthase gene homologue resides between the eryA homologous genes. A gene cluster of rubransarol and 2,6-dideoxynitrosugar is around 50 kb. Sequencing of the PCR product from the AHBA synthase gene homologue isolated from S. achromogenes revealed 85% amino acid sequence homology (73/86) with the AHBA synthase from a rifamycin-producer. dTDP-D-glucose 4,6-dehydratase gene homologue was subcloned from one of the isolated cosmid clones and sequenced. It showed 65% homology (43/66) with dTDP-D-glucose 4,6-dehydratase from a streptomycin-producer.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIdentification of a gene cluster of biosynthetic genes of rubradirin substructures in S. achromogenes var. rubradiris NRRL3061-
dc.title.alternativeIdentification of a gene cluster of biosynthetic genes of rubradirin substructures in S. achromogenes var. rubradiris NRRL3061-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number5-
dc.citation.endPage681-
dc.citation.startPage674-
dc.citation.volume7-
dc.contributor.affiliatedAuthorJung Joon Lee-
dc.contributor.alternativeName송재경-
dc.contributor.alternativeName오태진-
dc.contributor.alternativeName이정준-
dc.contributor.alternativeName김천규-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 7, no. 5, pp. 674-681-
dc.description.journalClassY-
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