DC Field | Value | Language |
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dc.contributor.author | Jae-Kyung Sohng | - |
dc.contributor.author | Tae-Jin Oh | - |
dc.contributor.author | Jung Joon Lee | - |
dc.contributor.author | Chun-Gyu Kim | - |
dc.date.accessioned | 2017-04-19T08:54:51Z | - |
dc.date.available | 2017-04-19T08:54:51Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4209 | - |
dc.description.abstract | Rubradirin, an ansamycin antibiotic has been purified from Streptomyces achromogenes var. rubradiris NRRL3061. It consists of four distinct structural moieties, rubransarol, 3-amino-4-hydroxy-coumarin, dihydroxydipicolinic acid, and 2,6-dideoxynitrosugar (DNS). Polymerase chain reaction (PCR) primers were designed based on consensus sequences of dTDP-D-glucose 4,6-dehydratase, one of enzymes involved in the biosynthesis of 2,6-dideoxysugar. A PCR product was obtained from S. achromogenes var. rubradiris. Hybridization of the PCR product to a cosmid library constructed from S. achromogenes genomic DNA has led to the identification of three unlinked regions of DNA. One of three kinds of cosmid clones contains homologues of dTDP-D-glucose 4,6-dehydratase, 3-amino-5-hydroxybenzoic acid (AHBA) synthase, and eryA genes. The size of the gene homologous to eryA is 30 kb, and the AHBA synthase gene homologue resides between the eryA homologous genes. A gene cluster of rubransarol and 2,6-dideoxynitrosugar is around 50 kb. Sequencing of the PCR product from the AHBA synthase gene homologue isolated from S. achromogenes revealed 85% amino acid sequence homology (73/86) with the AHBA synthase from a rifamycin-producer. dTDP-D-glucose 4,6-dehydratase gene homologue was subcloned from one of the isolated cosmid clones and sequenced. It showed 65% homology (43/66) with dTDP-D-glucose 4,6-dehydratase from a streptomycin-producer. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Identification of a gene cluster of biosynthetic genes of rubradirin substructures in S. achromogenes var. rubradiris NRRL3061 | - |
dc.title.alternative | Identification of a gene cluster of biosynthetic genes of rubradirin substructures in S. achromogenes var. rubradiris NRRL3061 | - |
dc.type | Article | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.number | 5 | - |
dc.citation.endPage | 681 | - |
dc.citation.startPage | 674 | - |
dc.citation.volume | 7 | - |
dc.contributor.affiliatedAuthor | Jung Joon Lee | - |
dc.contributor.alternativeName | 송재경 | - |
dc.contributor.alternativeName | 오태진 | - |
dc.contributor.alternativeName | 이정준 | - |
dc.contributor.alternativeName | 김천규 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, vol. 7, no. 5, pp. 674-681 | - |
dc.description.journalClass | Y | - |
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