DC Field | Value | Language |
---|---|---|
dc.contributor.author | Myung Ja Choi | - |
dc.contributor.author | Jeong Eun Choi | - |
dc.contributor.author | Do Young Yoon | - |
dc.contributor.author | Jong Sei Park | - |
dc.contributor.author | Sergei A Eremin | - |
dc.date.accessioned | 2017-04-19T08:54:59Z | - |
dc.date.available | 2017-04-19T08:54:59Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4246 | - |
dc.description.abstract | A homogeneous fluorescence polarization immunoassay (FPIA) was developed to measure levels of progesterone in urine using a TDx analyzer in photocheck mode (Abbott Labs). Two tracers of ethylenediamine fluorescein thiocarbamyl (EDF) were employed; one was synthesized from 11α-hydroxyhemisuccinate progesterone (Prog11OH-HS) and the other was synthesized from 3-(o- carboxymethyl)oxime progesterone (Prog-3CMO). Each derivative of progesterone was conjugated with bovine serum albumin and used as an immunogen which produced monoclonal antibody clone 15A (MAb 15A, anti-Prog-11OH-HS) and clone 2B7 (MAb 2B7, anti-Prog-3CMO), respectively. Different combinations of tracers and antibodies were investigated in the FPIA system. Similar sensitivity was observed when using the pair, MAb 2B7 and its homologous tracer, Prog-3CMO-EDF, or MAb 15A and its homologous tracer, Prog-11OH-HS- EDF. In this immunoassay, no separation step was required and the total time for an assay of 10 samples was approximately 7 min. The progesterone detection limit in a 10 μl sample was 3 ng/ml. The cross-reactivity results indicate that the A-, B- and D-ring of asteroid are buried in the binding pocket of MAb 15A, while the C-ring faced outward, resulting in cross- reactivity with 11-αhydroxy progesterone. The A-, B- and C-ring of asteroid of MAb 2B7, in contrast, are buried deep in the pocket leaving the D-ring facing outward, resulting in some different degrees of cross-reactivity with C17 position substituted steroids. | - |
dc.publisher | Pharmaceutical Soc Japan | - |
dc.title | Fluorescence polarization immunoassay of progesterone | - |
dc.title.alternative | Fluorescence polarization immunoassay of progesterone | - |
dc.type | Article | - |
dc.citation.title | Biological & Pharmaceutical Bulletin | - |
dc.citation.number | 4 | - |
dc.citation.endPage | 314 | - |
dc.citation.startPage | 309 | - |
dc.citation.volume | 20 | - |
dc.contributor.affiliatedAuthor | Do Young Yoon | - |
dc.contributor.alternativeName | 최명자 | - |
dc.contributor.alternativeName | 최정은 | - |
dc.contributor.alternativeName | 윤도영 | - |
dc.contributor.alternativeName | 박종세 | - |
dc.contributor.alternativeName | Eremin | - |
dc.identifier.bibliographicCitation | Biological & Pharmaceutical Bulletin, vol. 20, no. 4, pp. 309-314 | - |
dc.identifier.doi | 10.1248/bpb.20.309 | - |
dc.subject.keyword | fluorescence polarization immunoassay | - |
dc.subject.keyword | progesterone | - |
dc.subject.keyword | fluorescence | - |
dc.subject.keyword | immunoassay | - |
dc.subject.keyword | immunogen | - |
dc.subject.keyword | tracer | - |
dc.subject.local | fluorescence polarization immunoassay | - |
dc.subject.local | progesterone | - |
dc.subject.local | Progesterone | - |
dc.subject.local | fluorescence | - |
dc.subject.local | Fluorescence | - |
dc.subject.local | Immunoassay | - |
dc.subject.local | Immunoassays | - |
dc.subject.local | immunoassay | - |
dc.subject.local | immunogen | - |
dc.subject.local | tracer | - |
dc.description.journalClass | Y | - |
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