Fluorescence polarization immunoassay of progesterone

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dc.contributor.authorMyung Ja Choi-
dc.contributor.authorJeong Eun Choi-
dc.contributor.authorDo Young Yoon-
dc.contributor.authorJong Sei Park-
dc.contributor.authorSergei A Eremin-
dc.date.accessioned2017-04-19T08:54:59Z-
dc.date.available2017-04-19T08:54:59Z-
dc.date.issued1997-
dc.identifier.issn0918-6158-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4246-
dc.description.abstractA homogeneous fluorescence polarization immunoassay (FPIA) was developed to measure levels of progesterone in urine using a TDx analyzer in photocheck mode (Abbott Labs). Two tracers of ethylenediamine fluorescein thiocarbamyl (EDF) were employed; one was synthesized from 11α-hydroxyhemisuccinate progesterone (Prog11OH-HS) and the other was synthesized from 3-(o- carboxymethyl)oxime progesterone (Prog-3CMO). Each derivative of progesterone was conjugated with bovine serum albumin and used as an immunogen which produced monoclonal antibody clone 15A (MAb 15A, anti-Prog-11OH-HS) and clone 2B7 (MAb 2B7, anti-Prog-3CMO), respectively. Different combinations of tracers and antibodies were investigated in the FPIA system. Similar sensitivity was observed when using the pair, MAb 2B7 and its homologous tracer, Prog-3CMO-EDF, or MAb 15A and its homologous tracer, Prog-11OH-HS- EDF. In this immunoassay, no separation step was required and the total time for an assay of 10 samples was approximately 7 min. The progesterone detection limit in a 10 μl sample was 3 ng/ml. The cross-reactivity results indicate that the A-, B- and D-ring of asteroid are buried in the binding pocket of MAb 15A, while the C-ring faced outward, resulting in cross- reactivity with 11-αhydroxy progesterone. The A-, B- and C-ring of asteroid of MAb 2B7, in contrast, are buried deep in the pocket leaving the D-ring facing outward, resulting in some different degrees of cross-reactivity with C17 position substituted steroids.-
dc.publisherPharmaceutical Soc Japan-
dc.titleFluorescence polarization immunoassay of progesterone-
dc.title.alternativeFluorescence polarization immunoassay of progesterone-
dc.typeArticle-
dc.citation.titleBiological & Pharmaceutical Bulletin-
dc.citation.number4-
dc.citation.endPage314-
dc.citation.startPage309-
dc.citation.volume20-
dc.contributor.affiliatedAuthorDo Young Yoon-
dc.contributor.alternativeName최명자-
dc.contributor.alternativeName최정은-
dc.contributor.alternativeName윤도영-
dc.contributor.alternativeName박종세-
dc.contributor.alternativeNameEremin-
dc.identifier.bibliographicCitationBiological & Pharmaceutical Bulletin, vol. 20, no. 4, pp. 309-314-
dc.identifier.doi10.1248/bpb.20.309-
dc.subject.keywordfluorescence polarization immunoassay-
dc.subject.keywordprogesterone-
dc.subject.keywordfluorescence-
dc.subject.keywordimmunoassay-
dc.subject.keywordimmunogen-
dc.subject.keywordtracer-
dc.subject.localfluorescence polarization immunoassay-
dc.subject.localprogesterone-
dc.subject.localProgesterone-
dc.subject.localfluorescence-
dc.subject.localFluorescence-
dc.subject.localImmunoassay-
dc.subject.localImmunoassays-
dc.subject.localimmunoassay-
dc.subject.localimmunogen-
dc.subject.localtracer-
dc.description.journalClassY-
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