|dc.contributor.author||H D Paik||-|
|dc.contributor.author||S S Bae||-|
|dc.contributor.author||Seung Hwan Park||-|
|dc.contributor.author||Jae Gu Pan||-|
|dc.description.abstract||Bacillus thuringiensis subsp tochigiensis HD868 was identified as a bacteriocin producer which exhibited a bactericidal effect against closely related species. This bacteriocin designated as tochicin, was partially purified by 75% ammonium sulfate precipitation followed by subsequent dialysis. This partially purified tochicin showed a narrow antibacterial spectrum of activity against most of 20 typical B. thuringiensis strains and a strain of B. cereus, but not against other bacteria and yeasts tested. The antibacterial activity of tochicin on sensitive indicator cells disappeared completely by proteinase K treatment (1 mg ml-1), which indicates its proteinaceous nature. Tochicin was very stable throughout the range of pH 3.0-9.0 and was relatively heat-stable at 90°C, but bacteriocin activity was not detected after boiling for 30 min. The relationship between cell growth and bacteriocin production was studied in a semi-defined medium. Tochicin activity was detected at the mid-log growth phase, reached the maximum at the early stationary phase, but decreased after the stationary phase. Direct detection of tochicin activity on sodium dodecyl sulfate-polyacrylamide gel suggested it has an apparent molecular mass of about 10.5 kDa. Tochicin exhibited a bactericidal activity against B. thuringiensis subsp thompsoni HD522 in phosphate buffer (pH 7.0).||-|
|dc.title||Identification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp tochigiensis||-|
|dc.title.alternative||Identification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp tochigiensis||-|
|dc.citation.title||Journal of Industrial Microbiology & Biotechnology||-|
|dc.contributor.affiliatedAuthor||H D Paik||-|
|dc.contributor.affiliatedAuthor||S S Bae||-|
|dc.contributor.affiliatedAuthor||Seung Hwan Park||-|
|dc.contributor.affiliatedAuthor||Jae Gu Pan||-|
|dc.identifier.bibliographicCitation||Journal of Industrial Microbiology & Biotechnology, vol. 19, pp. 294-298||-|
There are no files associated with this item.
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.