Identification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp tochigiensis

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dc.contributor.authorH D Paik-
dc.contributor.authorS S Bae-
dc.contributor.authorSeung Hwan Park-
dc.contributor.authorJae Gu Pan-
dc.date.accessioned2017-04-19T08:55:00Z-
dc.date.available2017-04-19T08:55:00Z-
dc.date.issued1997-
dc.identifier.issn0169-4146-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4251-
dc.description.abstractBacillus thuringiensis subsp tochigiensis HD868 was identified as a bacteriocin producer which exhibited a bactericidal effect against closely related species. This bacteriocin designated as tochicin, was partially purified by 75% ammonium sulfate precipitation followed by subsequent dialysis. This partially purified tochicin showed a narrow antibacterial spectrum of activity against most of 20 typical B. thuringiensis strains and a strain of B. cereus, but not against other bacteria and yeasts tested. The antibacterial activity of tochicin on sensitive indicator cells disappeared completely by proteinase K treatment (1 mg ml-1), which indicates its proteinaceous nature. Tochicin was very stable throughout the range of pH 3.0-9.0 and was relatively heat-stable at 90°C, but bacteriocin activity was not detected after boiling for 30 min. The relationship between cell growth and bacteriocin production was studied in a semi-defined medium. Tochicin activity was detected at the mid-log growth phase, reached the maximum at the early stationary phase, but decreased after the stationary phase. Direct detection of tochicin activity on sodium dodecyl sulfate-polyacrylamide gel suggested it has an apparent molecular mass of about 10.5 kDa. Tochicin exhibited a bactericidal activity against B. thuringiensis subsp thompsoni HD522 in phosphate buffer (pH 7.0).-
dc.publisherSpringer-
dc.titleIdentification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp tochigiensis-
dc.title.alternativeIdentification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp tochigiensis-
dc.typeArticle-
dc.citation.titleJournal of Industrial Microbiology & Biotechnology-
dc.citation.number0-
dc.citation.endPage298-
dc.citation.startPage294-
dc.citation.volume19-
dc.contributor.affiliatedAuthorH D Paik-
dc.contributor.affiliatedAuthorS S Bae-
dc.contributor.affiliatedAuthorSeung Hwan Park-
dc.contributor.affiliatedAuthorJae Gu Pan-
dc.contributor.alternativeName백대헌-
dc.contributor.alternativeName배성숙-
dc.contributor.alternativeName박승환-
dc.contributor.alternativeName반재구-
dc.identifier.bibliographicCitationJournal of Industrial Microbiology & Biotechnology, vol. 19, pp. 294-298-
dc.identifier.doi10.1038/sj.jim.2900462-
dc.subject.keywordbacteriocin-
dc.subject.keywordBacillus thuringiensis-
dc.subject.keywordtochicin-
dc.subject.localBacteriocin-
dc.subject.localbacteriocins-
dc.subject.localbacteriocin-
dc.subject.localBacillus thuringiensis-
dc.subject.localbacillus thuringiensis-
dc.subject.localtochicin-
dc.description.journalClassY-
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