Genetically engineered human interleukin-6 variant with enhanced stability

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Title
Genetically engineered human interleukin-6 variant with enhanced stability
Author(s)
Sang Chul Lee; M J Lee; In Pyo Choi; Myung Hee Yu
Bibliographic Citation
Biotechnology Letters, vol. 19, no. 9, pp. 885-888
Publication Year
1997
Abstract
A recombinant human interleukin-6 mutant with enhanced conformational stability toward denaturant was obtained by site-specific mutagenesis. The clone was identified as having a single amino acid substitution of Lys70 → Glu. When urea-induced denaturation was monitored by the change in fluorescence intensity at 360 nm, Lys70 → Glu mutation shifted the midpoint of unfolding transition from 5.8 M (wild type) to 6.6 M urea. This mutation did not impair the biological activity.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1023/A:1018393721972
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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