Production and properties of a lipopeptide biosurfactant from Bacillus subtilis C9

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dc.contributor.authorHee Sik Kim-
dc.contributor.authorByung Dae Yoon-
dc.contributor.authorChang Ho Lee-
dc.contributor.authorHyun Hyo Suh-
dc.contributor.authorHee-Mock Oh-
dc.contributor.authorTohoru Katsuragi-
dc.contributor.authorYoshiki Tani-
dc.date.accessioned2017-04-19T08:55:01Z-
dc.date.available2017-04-19T08:55:01Z-
dc.date.issued1997-
dc.identifier.issn0385-6380-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4259-
dc.description.abstractA lipopeptide biosurfactant, C9-BS produced by Bacillus (B.) subtilis C9, emulsified hydrocarbons, vegetable oil and crude oil. A high yield of C9- BS was obtained from a culture of B. subtilis C9 using a carbohydrate substrate, while a hydrocarbon substrate inhibited the production of the biosurfactant. The optimum medium for the production of C9-BS was as follows (g/l): glucose, 40; NH4HCO3, 13.5; K2HPO4, 10.5; NaH2PO4, 1.5; MgSO4.7H2O, 0.5; MnSO4 · 4H2O, 0.05; yeast extract, 0.5. The initial pH was 8.0 and the culture temperature was 30°C. The biosurfactant C9-BS was obtained by collection of the foam that overflowed in the fermentor culture. The production of the biosurfactant by B. subtilis C9 was growth-associated. Under O2-limited conditions. C9-BS was produced in a 3-fold higher yield compared to that under O2-sufficient conditions. C9-BS is soluble in ethanol, acetone, methanol, butanol, chloroform, dichloromethane and alkaline water. The biosurfactant C9-BS lowered the surface tension of water to 28.5 dyne/cm, and the CMC (critical micelle concentration) was 40 μM. C9-BS was stable from pH 5.0 to pH 9.5, in incubation at 100°C for 1 h, and at a salt concentration of 1,000 mM for NaCl and 10 mM for CaCl2.-
dc.publisherSoc Bioscience Bioengineering Japan-
dc.titleProduction and properties of a lipopeptide biosurfactant from Bacillus subtilis C9-
dc.title.alternativeProduction and properties of a lipopeptide biosurfactant from Bacillus subtilis C9-
dc.typeArticle-
dc.citation.titleJournal of Fermentation Bioengineering-
dc.citation.number1-
dc.citation.endPage46-
dc.citation.startPage41-
dc.citation.volume84-
dc.contributor.affiliatedAuthorHee Sik Kim-
dc.contributor.affiliatedAuthorByung Dae Yoon-
dc.contributor.affiliatedAuthorChang Ho Lee-
dc.contributor.affiliatedAuthorHyun Hyo Suh-
dc.contributor.affiliatedAuthorHee-Mock Oh-
dc.contributor.alternativeName김희식-
dc.contributor.alternativeName윤병대-
dc.contributor.alternativeName이창호-
dc.contributor.alternativeName서현효-
dc.contributor.alternativeName오희목-
dc.contributor.alternativeNameKatsuragi-
dc.contributor.alternativeNameTani-
dc.identifier.bibliographicCitationJournal of Fermentation Bioengineering, vol. 84, no. 1, pp. 41-46-
dc.identifier.doi10.1016/S0922-338X(97)82784-5-
dc.subject.keywordbiosurfactant-
dc.subject.keywordlipopeptide-
dc.subject.keywordBacillus subtilis-
dc.subject.keywordproduction-
dc.subject.keywordproperties-
dc.subject.keywordstability-
dc.subject.localBiosurfactant-
dc.subject.localbiosurfactant-
dc.subject.localBiosurfactants-
dc.subject.locallipopeptide-
dc.subject.localLipopeptide-
dc.subject.locallipo-peptides-
dc.subject.localBacillus subtilis-
dc.subject.localbacillus subtilis-
dc.subject.localproduction-
dc.subject.localProduction-
dc.subject.localproperty-
dc.subject.localProperties-
dc.subject.localProperty-
dc.subject.localproperties-
dc.subject.localStability-
dc.subject.localstability-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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