Protein purification and cDNA cloning of a cecropin-like peptide from the larvae of fall webworm (Hyphantria cunea)

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Title
Protein purification and cDNA cloning of a cecropin-like peptide from the larvae of fall webworm (Hyphantria cunea)
Author(s)
Soon Sik Park; Sang Woon Shin; Doo Sang ParkHyun Woo Oh; Kyung Saeng Boo; Ho Yong Park
Bibliographic Citation
Insect Biochemistry and Molecular Biology, vol. 27, no. 8, pp. 711-720
Publication Year
1997
Abstract
A proteinous antimicrobial substance was purified from the bacteria- challenged larvae of the fall webworm, Hyphantria cunea. It is a cecropin- like antibacterial peptide which exhibits antibacterial activity against Gram-negative and Gram-positive bacteria, and known as Hyphantria cecropin A. The cDNA clones corresponding to this peptide were isolated from a cDNA library constructed from the bacteria-challenged larvae and obtained complete nucleotide sequences. In addition to the Hyphantria cecropin A sequence, we obtained three other cDNAs exhibiting high sequence similarity with Hyphantria cecropin A. We synthesized the C-terminally amidated peptide of 35 residues based on the deduced sequence of the isolated cDNA of Hyphantria cecropin A. The synthetic peptide exhibited strong antibacterial activity against several microbes including medically important bacteria such as Salmonella, Shigella, and fungus such as Candida. A Southern blot experiment using these cloned cDNAs as probes predicted the existence of multiple forms of Hyphantria cecropin genes.
Keyword
Insect immunityHyphantria cecropinFall webwormProtein purificationNucleotide sequence
ISSN
0965-1748
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/S0965-1748(97)00049-0
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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