Gas phase ethyl acetate production in a batch bioreactor

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Title
Gas phase ethyl acetate production in a batch bioreactor
Author(s)
Soon Ook Hwang; Young Hoon Park
Bibliographic Citation
Bioprocess Engineering, vol. 17, pp. 51-54
Publication Year
1997
Abstract
Gas phase ethyl acetate production was studied using a porcine pancreatic lipase powder. It was observed that gaseous ethyl acetate was produced from gaseous ethanol and acetic acid. Accordingly, the effects of amount of lipase powder, gaseous ethanol and acetic acid concentrations, and reaction temperature on the performance of a batch bioreactor were investigated. Apparent Michaelis-Menten constant of ethanol was 0.163 [μM] and there was no inhibition by ethanol over the range investigated. As acetic acid concentration increased, ethyl acetate production increased to a maximum, then decreased, thus suggesting the inhibition effects by acetic acid. Over the reaction temperature of 25-55 °C, activation energy was calculated as 3.93 kcal/gmol and initial reaction rate was obtained as follows: r - 75.7 exp(-1975.7/T) [μM/mg of lipase/hr].
ISSN
0178-515X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s004490050352
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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