Production of curdlan using sucrose or sugar cane molasses by two-step fed-batch cultivation of Agrobacterium species

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Title
Production of curdlan using sucrose or sugar cane molasses by two-step fed-batch cultivation of Agrobacterium species
Author(s)
In Young Lee; Weon Taek Seo; Guk Jin Kim; Mi Kyung Kim; C S Park; Young Hoon Park
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 18, pp. 255-259
Publication Year
1997
Abstract
Maltose and sucrose were efficient carbon sources for the production of curdlan by a strain of Agrobacterium sp. A two-step, fed-batch operation was designed in which biomass was first produced, followed by curdlan production which was stimulated by nitrogen limitation. There exists an optimal timing for nitrogen limitation for curdlan production in the two-step, fed-batch operation. Maximum curdlan production (60 g L-1) was obtained from sucrose with a productivity of 0.2 g L-1 h-1 when nitrogen was limited at a cell concentration of 16.0 g L-1. It was also noted that the curdlan yield from sucrose was as high as 0.45 g curdlan g-1 sucrose, and the highest specific production rate was 1.0 g curdlan g-1 cells h-1 right after nitrogen limitation. of particular importance was the use of molasses as a cheap carbon source to produce curdlan in the two-step, fed-batch cultivation. As high as 42 g L-1 of curdlan with a yield of 0.35 g curdlan g-1 total sugar was obtained after 120 h of fed-batch cultivation.
Keyword
curdlan productionAgrobacterium spnitrogen limitation
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1038/sj.jim.2900378
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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