Enhanced yield and a high production of medium-chain-length poly(3-hydroxyalkanoates) in a two-step fed-batch cultivation of Pseudomonas putida by combined use of glucose and octanoate

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dc.contributor.authorGuk Jin Kim-
dc.contributor.authorIn Young Lee-
dc.contributor.authorSung Chul Yoon-
dc.contributor.authorYong Chul Shin-
dc.contributor.authorYoung Hoon Park-
dc.date.accessioned2017-04-19T08:55:04Z-
dc.date.available2017-04-19T08:55:04Z-
dc.date.issued1997-
dc.identifier.issn0141-0229-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4283-
dc.description.abstractProduction of medium-chain-length (MCL) poly(3-hydroxyalkanoates) (PHAs) from a strain of Pseudomonas putida was studied. Although octanoate was found to be efficient for MCL PHA production, its yield for PHAs was low since it was also used for cell growth. A two-step fed-batch cultivation was thus designed with glucose and octanoate as the main carbon source for cell growth and PHA accumulation, respectively. After the cells were grown on glucose in the first step, they were subjected to nitrogen- and oxygen-limiting conditions in the second step in which octanoate was supplied as the sole carbon source. PHA (18.6 g l-1) was obtained with a yield of about 40% (g PHA g-1 octanoate). By using the same method but supplying octanoate in the first cell-growing step, production of MCL PHAs was significantly enhanced; it yielded 35.9 g PHA l-1 after 39 h of the fed-batch operation. The PHA content of the cell was also improved to 65.5% (g PHA g-1 cell mass) which indicated that octanoate addition stimulated quite efficiently the biosynthesis of MCL PHAs.-
dc.publisherElsevier-
dc.titleEnhanced yield and a high production of medium-chain-length poly(3-hydroxyalkanoates) in a two-step fed-batch cultivation of Pseudomonas putida by combined use of glucose and octanoate-
dc.title.alternativeEnhanced yield and a high production of medium-chain-length poly(3-hydroxyalkanoates) in a two-step fed-batch cultivation of Pseudomonas putida by combined use of glucose and octanoate-
dc.typeArticle-
dc.citation.titleEnzyme and Microbial Technology-
dc.citation.number7-
dc.citation.endPage505-
dc.citation.startPage500-
dc.citation.volume20-
dc.contributor.affiliatedAuthorGuk Jin Kim-
dc.contributor.affiliatedAuthorIn Young Lee-
dc.contributor.affiliatedAuthorYoung Hoon Park-
dc.contributor.alternativeName김국진-
dc.contributor.alternativeName이인영-
dc.contributor.alternativeName윤성철-
dc.contributor.alternativeName신영철-
dc.contributor.alternativeName박영훈-
dc.identifier.bibliographicCitationEnzyme and Microbial Technology, vol. 20, no. 7, pp. 500-505-
dc.identifier.doi10.1016/S0141-0229(96)00179-2-
dc.subject.keywordMedium-chain-length poly(3-hydroxyalkanoates)-
dc.subject.keywordProduction-
dc.subject.keywordPseudomonas putida-
dc.subject.localMedium-chain-length poly(3-hydroxyalkanoates)-
dc.subject.localproduction-
dc.subject.localProduction-
dc.subject.localPseudomonas putida-
dc.description.journalClassY-
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