Increased β-carotene synthesis in Blakeslea trispora under strong alkaline culture condition

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Title
Increased β-carotene synthesis in Blakeslea trispora under strong alkaline culture condition
Author(s)
Seon Won Kim; Weon Taek Seo; Young Hoon Park
Bibliographic Citation
Biotechnology Letters, vol. 18, no. 11, pp. 1287-1290
Publication Year
1996
Abstract
A high yield of β-carotene, 48 mg/l, was obtained by cultivation of a strain of Blakeslea trispora under a strong alkaline culture condition (pH 10), which was significantly higher than those obtained under milder pH conditions (3.26 - 7.80 mg/l at pH 4 to 8). The formation of small pellets at pH 10 was observed, whereas a single large cluster was formed for the pHs ranged from 4 to 8. Span 20 at 0.1 to 1% in the culture medium changed cellular morphology to micro-pellets regardless of the culture pH. The same trend of β-carotene production, depending on the alkalinity of the culture broth, was also observed in the presence of 1% of Span 20 yielding much higher productivity. The amounts of β-carotene yielded at pH 10 and 11 were 92.0 and 100.9 mg/l, respectively, which were about two times higher than those obtained at pH 4 to 9.
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/BF00129956
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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