Cloning of the human cDNA sequence encoding the NADH : ubiquinone oxidoreductase MLRQ subunit

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Title
Cloning of the human cDNA sequence encoding the NADH : ubiquinone oxidoreductase MLRQ subunit
Author(s)
Jae Wha Kim; Yong Hee Lee; Ho Bum Kang; Young Kyung Choe; Tae Wha Chung; Sung Yeoul Chang; Kwang Soo Lee; In Seong Choe
Bibliographic Citation
Biochemistry and Molecular Biology International, vol. 43, no. 3, pp. 669-675
Publication Year
1997
Abstract
A cDNA clone encoding human NADH:ubiquinone oxidoreductase (complex I of mitochondrial respiratory chain) MLRQ subunit was isolated from human fetal liver cDNA library. The clone contained an open reading frame of 246 bp which predicted a protein comprising 81 amino acids with a calculated molecular weight of 9370 Da. The deduced amino acid sequence exhibited 95% homology (88% identity and 7% favored substitution) to that of bovine MLRQ subunit. Northern analysis revealed that the cDNA clone hybridized with a 0.7 kb mRNA species which was present in all tissues examined. The expression level of the 0.7 kb mRNA in heart, skeletal muscle, and brain was higher than in other organs. Human MLRQ cDNA could cross-hybridize with the genomic DNAs from various species.
Keyword
NADH:ubiquinone oxidoreductaseMitochondrial respiratory chainMLRQ subunitcDNA sequenceTissue distribution(Human)
ISSN
1039-9712
Publisher
Wiley
DOI
http://dx.doi.org/10.1080/15216549700204471
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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