The bacillus subtilis genome sequencing project in Korea: sequence ananlysis of the 53kb DNA fragment at 180˚-185˚ of B. subtilis 168 chromosome = 한국에서의 고쵸균 유전체 연구: Bacillus subtilis 염색체상 180˚-185˚ 부위 53 kb DNA 단편의 염기서열 분석

Abstract

The entire sequence of a 4,214,810 bp genome of the Bacillus subtilis 168 has been determined by an international project, and the completion has been announced on July 19, 1997. For the sequencing project an international consortium was established and 25 European, 7 Japanese laboratories, 2 biotechnology companies, and our laboratory participated in the project. Within this framework we determined the complete nucleotide sequence of a 53,289 bp fragment upstream of the odhA gene (181 °) of the B. subtilis 168 chromosome. On the basis of the published DNA sequences of the B. subtilis sspC and odhA genes, we obtained genomic fragments by plasmid rescue and long-range PCR. The sequenced fragment contains 56 putative open reading frames (designated yojA-yoll) and 9 known genes (sspC, cge cluster, orfE5, orfRM1 and odhA), in which we found many interesting features. In addition, the entire nucleotide sequence of a 53,289 bp region enabled us to revise the current genetic map of this region.