Development of a program for fragment assembly from DNA sequence data = DNA 염기 서열의 단편 조립 프로그램 개발

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Title
Development of a program for fragment assembly from DNA sequence data = DNA 염기 서열의 단편 조립 프로그램 개발
Author(s)
Byung Uk Lee; Kie Jung Park; Wan Park; Yong Ha Park
Bibliographic Citation
Korean Journal of (Applied) Microbiology & Biotechnology, vol. 25, no. 6, pp. 560-565
Publication Year
1997
Abstract
DNA fragment assembly is a major concern in shot-gun DNA sequencing project. It is to reconstruct a consensus DNA sequence from a collection of random oritented fragments. We developed a computer program that is useful for DNA fragment assembly. Inputs to the program are DNA fragment sequences including IUB-IUPAC bases. The program produces the most probable reconstruction of the original DNA sequence as a text format or a PostScript format. The program consists of four phases: the first phase quickly eliminates fragment pairs that can not possibly overlap. In the second phase, the quality of overlap between each pair is calculated to a score. In the third phase, overlap pairs are sorted by their scores and consistency of the overlaps is checked. The last phase determines consensus sequences and displays them. The performance of fragment assembly program was tested on a set of DNA fragment sequences which were generated from long DNA sequences of GenBank by a fragmentation program.
Keyword
ContingDNA assembly programDNA fragmentIUB-IUPAC base
ISSN
0257-2389
Publisher
Korea Soc-Assoc-Inst
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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