Isolation of synthetic lethal mutants of ras1 in Schizosaccharomyces pombe

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorKyung Sook Chung-
dc.contributor.authorKyu Won Kim-
dc.contributor.authorHyang Sook Yoo-
dc.date.accessioned2017-04-19T08:55:09Z-
dc.date.available2017-04-19T08:55:09Z-
dc.date.issued1997-
dc.identifier.issn1016-8478-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4318-
dc.description.abstractRas proteins are membrane-associated guanine nucleotide-binding proteins that serve as molecular switches for signal transduction pathways in a diverse array of organisms. Various cellular factors are known to interact with Ras proteins. In order to find the novel cellular factors that are associated with Ras function, we have constructed synthetic lethal mutants of the ras1+ gene in Schizosaccharomyces pombe and used them to identify the genes that are functionally dependent on the Ras1. We first constructed S. pombe strains in which chromosomal ras1 gene is placed under the nmt1 promoter that is regulated by thiamine. This strain shows ras1+ phenotype in the absence of thiamine, whereas it shows ras1- phenotype in the presence of thiamine. Second, we mutated the constructed strains with ultraviolet light (UV) and selected two synthetic lethal mutants that could not grow when Ras1 function was repressed (ras1-). One of the mutants, KSC3, showed a swollen cell shape, aberrant deposition of septum materials, and aberrant nuclei. The other mutant, KSC4, showed sensitivity to hyper-osmolarity when Ras1 function is absent. These mutants, however, grow normally when Ras1 is expressed (ras1+). These two novel synthetic lethal mutants of ras1 provide the means to isolate the corresponding genes that function in association with Ras1 in S. pombe. Screening of a genomic library of S. pombe complementing the mutant phenotype allowed us to identify several novel genes associated with Ras1 of S. pombe.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleIsolation of synthetic lethal mutants of ras1 in Schizosaccharomyces pombe-
dc.title.alternativeIsolation of synthetic lethal mutants of ras1 in Schizosaccharomyces pombe-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number6-
dc.citation.endPage806-
dc.citation.startPage800-
dc.citation.volume7-
dc.contributor.affiliatedAuthorKyung Sook Chung-
dc.contributor.affiliatedAuthorHyang Sook Yoo-
dc.contributor.alternativeName정경숙-
dc.contributor.alternativeName김규원-
dc.contributor.alternativeName유향숙-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 7, no. 6, pp. 800-806-
dc.subject.keywordNMT1 protein, S pombe-
dc.subject.keywordRas1 protein, S pombe-
dc.subject.keywordSchizosaccharomyces pombe protein-
dc.subject.keywordSchizosaccharomyces-
dc.subject.localNMT1 protein, S pombe-
dc.subject.localRas1 protein, S pombe-
dc.subject.localSchizosaccharomyces pombe protein-
dc.subject.localSchizosaccharomyces-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.