Cited 3 time in
- Expression of recombinant human inteleukin 6(rhIL6) in Saccharomyces cerevisiae by the midified phosphoglycerate kinase and chelatin promoter
- Kyung Sook Chung; Hyung Sik Kang; Kyu Won Kim; In Pyo Choi; Kwang Ho Pyun; Hyang Sook Yoo
- Bibliographic Citation
- Biotechnology Letters, vol. 19, no. 12, pp. 1169-1173
- Publication Year
- Using the modified yeast expression vectors that contained phosphoglycerate kinase or chelatin promoter with the adenine (A) base at -3 position from the ATG coding sequences and the leu-2 gene, recombinant human interleukin-6 (rhIL-6) was produced as a β-galactosidase (lacZ) fusion protein in Saccharomyces cerevisiae. Expression level of the IL-6-lacZ was 12 times higher than that from the unmodified wild type plasmids and IL-6 activity was 9-9.8 x 105 unit/mg yeast total protein. Thus these modified yeast vectors are useful for high expression of foreign genes in yeast.
- Appears in Collections:
- Division of Research on National Challenges > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
- Files in This Item:
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.