Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae

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dc.contributor.authorHyun Ah Kang-
dc.contributor.authorMoon Soo Jung-
dc.contributor.authorWon Kyoung Hong-
dc.contributor.authorJung Hoon Sohn-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorSang Ki Rhee-
dc.date.accessioned2017-04-19T08:55:10Z-
dc.date.available2017-04-19T08:55:10Z-
dc.date.issued1998-
dc.identifier.issn1017-7825-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4326-
dc.description.abstractIn order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GAL10, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleExpression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae-
dc.title.alternativeExpression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae-
dc.typeArticle-
dc.citation.titleJournal of Microbiology and Biotechnology-
dc.citation.number1-
dc.citation.endPage48-
dc.citation.startPage42-
dc.citation.volume8-
dc.contributor.affiliatedAuthorHyun Ah Kang-
dc.contributor.affiliatedAuthorWon Kyoung Hong-
dc.contributor.affiliatedAuthorJung Hoon Sohn-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.affiliatedAuthorSang Ki Rhee-
dc.contributor.alternativeName강현아-
dc.contributor.alternativeName정문수-
dc.contributor.alternativeName홍원경-
dc.contributor.alternativeName손정훈-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName이상기-
dc.identifier.bibliographicCitationJournal of Microbiology and Biotechnology, vol. 8, no. 1, pp. 42-48-
dc.subject.keywordSaccharomyces cerevisiae-
dc.subject.keywordhuman serum albumin-
dc.subject.keywordsecretion-
dc.subject.keywordprotein expression-
dc.subject.keywordprotein secretion-
dc.subject.localSaccharomyces cerevisiae-
dc.subject.localsaccharomyces cerevisiae-
dc.subject.localHuman serum albumin-
dc.subject.localhuman serum albumin-
dc.subject.localSecretion-
dc.subject.localsecretion-
dc.subject.localProtein expression-
dc.subject.localprotein expression-
dc.subject.localProtein Expression-
dc.subject.localProtein secretion-
dc.subject.localprotein secretion-
dc.description.journalClassY-
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Synthetic Biology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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