DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hyun Ah Kang | - |
dc.contributor.author | Moon Soo Jung | - |
dc.contributor.author | Won Kyoung Hong | - |
dc.contributor.author | Jung Hoon Sohn | - |
dc.contributor.author | Eui Sung Choi | - |
dc.contributor.author | Sang Ki Rhee | - |
dc.date.accessioned | 2017-04-19T08:55:10Z | - |
dc.date.available | 2017-04-19T08:55:10Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4326 | - |
dc.description.abstract | In order to maximize the secretory expression of human serum albumin (HSA) in the yeast Saccharomyces cerevisiae, a series of HSA expression vectors were constructed with a combination of different promoters, 5' untranslated regions (5'UTR), and secretion signal sequences. The expression vector composed of the galactose-inducible promoter GAL10, the natural 5'UTR, and the natural signal sequence of HSA directed the most efficient expression and secretion of HSA among the constructed vectors when introduced into several S. cerevisiae strains. Although the major form of HSA expressed and secreted in the yeast transformants was the mature form of 66 kDa, the truncated form of 45 kDa was also detected both in the cell extract and in the culture supernatant. The level of the intact HSA protein in the culture supernatant reached up to 30 mg/l at 24 h of cultivation in a shake-flask culture but began to decrease afterwards, indicating that the secreted HSA protein was unstable in a prolonged culture of yeast. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae | - |
dc.title.alternative | Expression and secretion of human serum albumin in the yeast Saccharomyces cerevisiae | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 1 | - |
dc.citation.endPage | 48 | - |
dc.citation.startPage | 42 | - |
dc.citation.volume | 8 | - |
dc.contributor.affiliatedAuthor | Hyun Ah Kang | - |
dc.contributor.affiliatedAuthor | Won Kyoung Hong | - |
dc.contributor.affiliatedAuthor | Jung Hoon Sohn | - |
dc.contributor.affiliatedAuthor | Eui Sung Choi | - |
dc.contributor.affiliatedAuthor | Sang Ki Rhee | - |
dc.contributor.alternativeName | 강현아 | - |
dc.contributor.alternativeName | 정문수 | - |
dc.contributor.alternativeName | 홍원경 | - |
dc.contributor.alternativeName | 손정훈 | - |
dc.contributor.alternativeName | 최의성 | - |
dc.contributor.alternativeName | 이상기 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 8, no. 1, pp. 42-48 | - |
dc.subject.keyword | Saccharomyces cerevisiae | - |
dc.subject.keyword | human serum albumin | - |
dc.subject.keyword | secretion | - |
dc.subject.keyword | protein expression | - |
dc.subject.keyword | protein secretion | - |
dc.subject.local | Saccharomyces cerevisiae | - |
dc.subject.local | saccharomyces cerevisiae | - |
dc.subject.local | Human serum albumin | - |
dc.subject.local | human serum albumin | - |
dc.subject.local | Secretion | - |
dc.subject.local | secretion | - |
dc.subject.local | Protein expression | - |
dc.subject.local | protein expression | - |
dc.subject.local | Protein Expression | - |
dc.subject.local | Protein secretion | - |
dc.subject.local | protein secretion | - |
dc.description.journalClass | Y | - |
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