Cloning and phylogenetic analysis of chitin synthase genes from the insect pathogenic fungus, Metarhizium anisopliae var. anisopliae

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dc.contributor.authorJin Sik Nam-
dc.contributor.authorDong Hun Lee-
dc.contributor.authorKang Hyun Lee-
dc.contributor.authorHee Moon Park-
dc.contributor.authorKyung Sook Bae-
dc.date.accessioned2017-04-19T08:55:10Z-
dc.date.available2017-04-19T08:55:10Z-
dc.date.issued1998-
dc.identifier.issn0378-1097-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4329-
dc.description.abstractDegenerated PCR primers were used to amplify chitin synthase genes from genomic DNA of Metarhizium anisopliae var. anisopliae. Through cloning and sequencing of approximately 600-bp fragments amplified by PCR, we found three genes encoding different types of chitin synthases, designated MaCHS1, MaCHS2, and MaCHS3. Southern blot analysis performed on genomic DNA showed that each of the chitin synthases MaCHS1, MaCHS2, and MnCHS3 is encoded by a single copy gene. Alignment of their deduced amino acid sequences with those of other euascomycetes separated the sequences into three distinct classes, MaCHS1 was identified as a gene for class I chitin synthase, MaCHS2 for class II, and MaCHS3 for class III. The UPGMA dendrogram and phylogenetic tree of the deduced amino acid sequences revealed the taxonomic and evolutionary position of Metarhizium anisopliae var. anisopliae.-
dc.publisherOxford Univ Press-
dc.titleCloning and phylogenetic analysis of chitin synthase genes from the insect pathogenic fungus, Metarhizium anisopliae var. anisopliae-
dc.title.alternativeCloning and phylogenetic analysis of chitin synthase genes from the insect pathogenic fungus, Metarhizium anisopliae var. anisopliae-
dc.typeArticle-
dc.citation.titleFEMS Microbiology Letters-
dc.citation.number1-
dc.citation.endPage84-
dc.citation.startPage77-
dc.citation.volume159-
dc.contributor.affiliatedAuthorJin Sik Nam-
dc.contributor.affiliatedAuthorKang Hyun Lee-
dc.contributor.affiliatedAuthorKyung Sook Bae-
dc.contributor.alternativeName남진식-
dc.contributor.alternativeName이동훈-
dc.contributor.alternativeName이강현-
dc.contributor.alternativeName박희문-
dc.contributor.alternativeName배경숙-
dc.identifier.bibliographicCitationFEMS Microbiology Letters, vol. 159, no. 1, pp. 77-84-
dc.identifier.doi10.1111/j.1574-6968.1998.tb12844.x-
dc.subject.keywordMetarhizium anisopliae var. anisopliae-
dc.subject.keywordChitin synthase gene-
dc.subject.keywordEntomopathogenic fungus-
dc.subject.keywordPhylogeny-
dc.subject.localMetarhizium anisopliae var. anisopliae-
dc.subject.localChitin synthase genes-
dc.subject.localchitin synthase gene-
dc.subject.localChitin synthase gene-
dc.subject.localEntomopathogenic fungus-
dc.subject.localphylogeny-
dc.subject.localPhylogeny-
dc.description.journalClassY-
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Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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