In situ hybridization법과 중합효소 연쇄반응을 이용한 한국여성 자궁경부내 papilloma 바이러스 감염의 검색

Abstract

The relationship of certain types of human papillomavirus(HPV) infections and cervical cancers has been strongly confined. HPV types such as 6b and 11 are associated with benign tumors such as condylomas, while HPV types 16, 18, and occasionally, 31, 33, 51 types are assoeiated with cervical intraepithelial neoplasia(CIN) and carcinoma. Especially HPV 16 and 18 types are frequently found in malignan tleisons of cervical tissues. In this study, we examined and compared the prevalence of several types of HPVs in cervical tissues frorn Korean women using in situ hybridzation (ISH) and DNA polymerase chain reaction (PCR). Fifty cases of paraffin-embedded in only one of 14 cases with low and high grade CINs, HPV 16/18 types in 3(21% ) of 14 with low and high grade CINs, 6(24%) of 25 with CIS, and none of 4 with invasive carcinoma, and HPV types 31/33/51 in 2(14%) of 14 with low and high grade CIN a, 6(25 %) of 25 with CIS, and none of 4 with invasive carcinoma by ISH. Fifty-six cases of paraffin-embedded tissues and another 10 more tissues stored in liquid nitrogen were analyzed by PCR. When the presence of HPV 16 genome was examined by the amplification of 16 type E6 oncogene, 37/55(67%) and 6(60% ) cases were positive for paraffin-embedded tissues and frozen tissues, respectively. When the positivity for the presence of HPV 16 E6 genome in thirty-six tissues processed both for ISH, 9/36 (25% ) and PCR, 26/36 (72% ) methods was compared, the latter was extremely sensitive. When the presence of HPV Ll gene coding for major capsid antigen was amplified by using Ll consensus amplimers for paraffin embedded tissues, 28 samples of 45(62%) were found to be positive, 21 of them were HPV 16-positive, while for frozen tissues 6 of 10 were HPV 16-positive.This implies that other types of HPVs are presentin 7 and 2 cases of them, respectively. The amplified DNAs by PCR were confirmed by Southern blot hybridization using either P-labelled of biotin-labelled probe.