Streptomyces 배양액에서의 interleukin-1 유사물질의 동정

Abstract

We have identified a T cell-activating material in the culture supernatant of Streptomyces species. The factor in microbial culture supernatant (MCS) induced thymocyte proliferation in a dose dependent fashion and it could be detected by immunoblot analysis using anti-interleukin1(IL-1) antibody. The factor in MCS was slightly larger(about 21 kd) in its molecular weight than IL-1 on SDS-PAGE. When ^125I-MCS was covalently coupled with homo-bifunctional cross-linking agent, disuccinimidyl-propionate to IL-1 receptor(IL-1R) on mouse thymoma cell(EL-4) and immunoprecipitated with anti-IL-1R antibody the molecular weight of this complex of 110 kd was observed. Interaction between IL-1 receptor and the factor in MCS could be inhibited by addition of excess IL- lβ. These results suggest that Streptomyces species may produce a factor which is closely related to IL-1β in its functional and biochemical characteristics.