재조합 preS2 peptide에 대한 단일 클론항체 생산과 그를 이용한 면역 친화성 column 제조에 관한 연구

Cited 0 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorMi Young Han-
dc.contributor.authorSang Hoon Choi-
dc.contributor.authorIn Seong Choe-
dc.contributor.authorMyung Ja Choi-
dc.contributor.authorTai Wha Chung-
dc.date.accessioned2017-04-19T08:55:17Z-
dc.date.available2017-04-19T08:55:17Z-
dc.date.issued1989-
dc.identifier.issn0368-4881-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4363-
dc.description.abstractThree monoclonal antibodies specific to the recombinant preS₂ peptide were prepared using preS₂-β-galactosidase as an immunogen. All three were classified as immunoglobulin M. One of the three monoclonal antibodies (040-01) was precipitated by 6% PEG and used for the preparation of immunoadsorbent column. This immunoaffinity column was utilized to purify recombinant preS₂ peptide which was solubilized and extracted from sonicated E. coli cells. For the storage of this specific antibody, 0.1 M glycine-HCl buffer (pH 2.5) gave the best result. Other buffers tested in this study caused a significant decrease in antibody reactivity. The binding capacity of an immunoadsorbent column for preS₂ peptide was determined using preS₂-ABEI-H conjugate.-
dc.publisherKorea Soc-Assoc-Inst-
dc.title재조합 preS2 peptide에 대한 단일 클론항체 생산과 그를 이용한 면역 친화성 column 제조에 관한 연구-
dc.title.alternativeProduction of anti-preS2 peptide monoclonal antibodies and preparation of anti-preS2 immunoaffinity column-
dc.typeArticle-
dc.citation.titleKorean Biochemical Journal-
dc.citation.number3-
dc.citation.endPage338-
dc.citation.startPage332-
dc.citation.volume22-
dc.contributor.affiliatedAuthorMi Young Han-
dc.contributor.affiliatedAuthorIn Seong Choe-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.alternativeName한미영-
dc.contributor.alternativeName최상훈-
dc.contributor.alternativeName최인성-
dc.contributor.alternativeName최명자-
dc.contributor.alternativeName정태화-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 22, no. 3, pp. 332-338-
dc.description.journalClassN-
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.