DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mi Young Han | - |
dc.contributor.author | Sang Hoon Choi | - |
dc.contributor.author | In Seong Choe | - |
dc.contributor.author | Myung Ja Choi | - |
dc.contributor.author | Tai Wha Chung | - |
dc.date.accessioned | 2017-04-19T08:55:17Z | - |
dc.date.available | 2017-04-19T08:55:17Z | - |
dc.date.issued | 1989 | - |
dc.identifier.issn | 0368-4881 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4363 | - |
dc.description.abstract | Three monoclonal antibodies specific to the recombinant preS₂ peptide were prepared using preS₂-β-galactosidase as an immunogen. All three were classified as immunoglobulin M. One of the three monoclonal antibodies (040-01) was precipitated by 6% PEG and used for the preparation of immunoadsorbent column. This immunoaffinity column was utilized to purify recombinant preS₂ peptide which was solubilized and extracted from sonicated E. coli cells. For the storage of this specific antibody, 0.1 M glycine-HCl buffer (pH 2.5) gave the best result. Other buffers tested in this study caused a significant decrease in antibody reactivity. The binding capacity of an immunoadsorbent column for preS₂ peptide was determined using preS₂-ABEI-H conjugate. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | 재조합 preS2 peptide에 대한 단일 클론항체 생산과 그를 이용한 면역 친화성 column 제조에 관한 연구 | - |
dc.title.alternative | Production of anti-preS2 peptide monoclonal antibodies and preparation of anti-preS2 immunoaffinity column | - |
dc.type | Article | - |
dc.citation.title | Korean Biochemical Journal | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 338 | - |
dc.citation.startPage | 332 | - |
dc.citation.volume | 22 | - |
dc.contributor.affiliatedAuthor | Mi Young Han | - |
dc.contributor.affiliatedAuthor | In Seong Choe | - |
dc.contributor.affiliatedAuthor | Tai Wha Chung | - |
dc.contributor.alternativeName | 한미영 | - |
dc.contributor.alternativeName | 최상훈 | - |
dc.contributor.alternativeName | 최인성 | - |
dc.contributor.alternativeName | 최명자 | - |
dc.contributor.alternativeName | 정태화 | - |
dc.identifier.bibliographicCitation | Korean Biochemical Journal, vol. 22, no. 3, pp. 332-338 | - |
dc.description.journalClass | N | - |
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