E. coli에서 사람 프리트롬빈 2 cDNA의 크로닝과 발현

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dc.contributor.authorEun Hwa Choi-
dc.contributor.authorYong Joo Kim-
dc.contributor.authorJong Myoung Kim-
dc.contributor.authorHyo Jeong Hong-
dc.contributor.authorMoon Hi Han-
dc.contributor.authorJi Young Kim-
dc.date.accessioned2017-04-19T08:55:17Z-
dc.date.available2017-04-19T08:55:17Z-
dc.date.issued1989-
dc.identifier.issn03684881-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4366-
dc.description.abstractA 0.6 kbp of 3'-portion of human prothrombin cDNA was isolated from a liver cDNA library of ${\lambda}gt$ 10 by in situ plaque hybridization. A cDNA fragment corresponding to the N-terminal region of prethrombin 2 was obtained from another cDNA library synthesized by reverse transcription of human liver mRNA using a specific oligonucleotide as a primer. The 48 bp cDNA sequences starting from the first nucleotide to the AvaI site of human prethrombin 2 cDNA was chemically synthesized such that prethrombin 2 can be expressed directly in E. coli expression vector system. The open reading frame(ORF) for human prethrombin 2 was cloned in pUC18 by combining the synthetic fragment and the cDNA fragments. Expression plasmids were constructed in which the ORF for human prethrombin 2 was inserted into the site downstream from the thermoinducible leftward promoter $(P_L)$ and the cII ribosome binding site of bacteriophage ${\lambda}$. Upon temperature induction, the plasmids expressed 34,000 dalton polypeptide, which was accumulated to an amount corresponding to 5-10% of the total bacterial proteins. The 34,000 dalton polypeptide was identified as human prethrombin 2 by SDS-gel electrophoresis and Western blot analysis.-
dc.publisherSouth Korea-
dc.titleE. coli에서 사람 프리트롬빈 2 cDNA의 크로닝과 발현-
dc.title.alternativeCloning and expression of human prethrombin 2 cDNA in Escherichia coli-
dc.typeArticle-
dc.citation.titleKorean Biochemical Journal-
dc.citation.number2-
dc.citation.endPage160-
dc.citation.startPage154-
dc.citation.volume22-
dc.contributor.alternativeName최은화-
dc.contributor.alternativeName김영주-
dc.contributor.alternativeName김종명-
dc.contributor.alternativeName홍효정-
dc.contributor.alternativeName한문희-
dc.contributor.alternativeName김지영-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 22, no. 2, pp. 154-160-
dc.description.journalClassN-
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