Overproduction of human interleukin-2 in E. coli

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dc.contributor.authorSeong Man Kang-
dc.contributor.authorSung Wan Kim-
dc.contributor.authorHyun Jung Ha-
dc.contributor.authorDoe Sun Na-
dc.contributor.authorSue Nie Park-
dc.contributor.authorJi Young Kim-
dc.contributor.authorMoon Hi Han-
dc.date.accessioned2017-04-19T08:55:17Z-
dc.date.available2017-04-19T08:55:17Z-
dc.date.issued1988-
dc.identifier.issn0368-4881-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4369-
dc.description.abstractIn order to increase the productivity of human IL-2 (interleukin-2), a stoichiometric model has been used to determine the most essential amino acids and precise values of their amounts to be added to the culture during expression of human IL-2 (as a model protein) by recombinant Escherichia coli BL21 (pET21a-hil2). Experiments were performed to investigate the effect of chosen amino acids and their interactions on expression of human IL-2. Glutamine, a mixture of leucine, aspartic acid and glycine, and a mixture of leucine, glutamine and aspartic acid, were the most effective for the expression of IL-2. The most promising amino acids were then chosen for further experiments at three different levels to determine whether altering their stoichiometry can lead to better expression levels. The optimized value of glutamine in the flask was 0.316 g/l; a mixture of leucine, glutamine and aspartic acid at concentrations of 0.124, 0.316 and 0.212 g/l respectively and of leucine, aspartic acid and glycine in concentrations of 0.124, 0.212, 0.111 g/l respectively were chosen to be added to the flask. The effect of glutamine, as one of the amino acids most influencing the expression of IL-2 in batch and fed-batch high-cell-density cultures, was studied. The results revealed that the amount of expressed IL-2 compared with the control culture increased from 81 to 195 mg/l in the shake flask, 403 to 594 mg/l in the fermentor and 5.15 to 10.01 g/l in the fermentor under fed-batch cultivation.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleOverproduction of human interleukin-2 in E. coli-
dc.title.alternativeOverproduction of human interleukin-2 in E. coli-
dc.typeArticle-
dc.citation.titleKorean Biochemical Journal-
dc.citation.number1-
dc.citation.endPage45-
dc.citation.startPage38-
dc.citation.volume21-
dc.contributor.affiliatedAuthorSue Nie Park-
dc.contributor.affiliatedAuthorMoon Hi Han-
dc.contributor.alternativeName강성만-
dc.contributor.alternativeName김성완-
dc.contributor.alternativeName하현정-
dc.contributor.alternativeName나도선-
dc.contributor.alternativeName박순희-
dc.contributor.alternativeName김지영-
dc.contributor.alternativeName한문희-
dc.identifier.bibliographicCitationKorean Biochemical Journal, vol. 21, no. 1, pp. 38-45-
dc.identifier.doi10.1042/BA20080300-
dc.description.journalClassN-
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