The evaluation of T/E and T/LH ratios in athelete's urine by the radioimmunoassay methods

Abstract

The studies reported that the urinary ratios of testosterone/epitestosterone(T/E) and testosterone/leutenizing horomone(T/LH) were increased significantly by the administration of anabolic steroids to normal subjects. Based on the report above, the T/E and T/LH ratiod were investigated by a RIA method for the 194 urine specimens of the Asian Games atheletes. The normal distribution of the restosterone(T), epotestosterone(E). and leutenizing hormone(LH) values were established and the T/E and T/LH ratios were investigated for steroid doping control. The correlation study between the T/E and T/LH ratios indicates that the high T/E ratio is not necessarily associated with the high T/LH ratio within the population who have the normal values of the T/E and T/LH ratios. It was also found that the RIA performances of T and E assays were very sensitive and could quantitatively detect the T and E respectively without cross-reaction. the standard curve range covers a broad range of steroid values.