Cloning and expression of a clostridium thermocellum xylanase gene in Escherichia coli

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dc.contributor.authorKyung Hwa Jung-
dc.contributor.authorKang Moon Lee-
dc.contributor.authorHoon Kim-
dc.contributor.authorKi Hong Yoon-
dc.contributor.authorSeung Hwan Park-
dc.contributor.authorMoo Young Pack-
dc.date.accessioned2017-04-19T08:55:19Z-
dc.date.available2017-04-19T08:55:19Z-
dc.date.issued1998-
dc.identifier.issn1039-9712-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4380-
dc.description.abstractA Clostridium thermocellum xylanase gene, designated xynX, was cloned in Escherichia coli and was catagorized a novel gene as a result of the comparison of restriction patterns of the C. thermocellum xylanase genes so far reported. The xynX gene encodes a xylanase having the molecular weight of 105 kilodaltons. A number of smaller truncated proteins with activities towards 4-methylumbelliferyl-β-D-cellobioside and xylan were also produced. The enzyme hydrolyzed xylan to xylo-oligosaccharide, indicating typical activity of endo-β-1,4-xylanase. This endoxylanase hydrolyzed carboxymethylcellulose without notable reduction of the viscosity as an exo-β-1,4-glucanase, even though the enzyme exhibited very low levels of activity against other soluble and insoluble cellulosic substrates.-
dc.publisherWiley-
dc.titleCloning and expression of a clostridium thermocellum xylanase gene in Escherichia coli-
dc.title.alternativeCloning and expression of a clostridium thermocellum xylanase gene in Escherichia coli-
dc.typeArticle-
dc.citation.titleBiochemistry and Molecular Biology International-
dc.citation.number2-
dc.citation.endPage292-
dc.citation.startPage283-
dc.citation.volume44-
dc.contributor.affiliatedAuthorKyung Hwa Jung-
dc.contributor.affiliatedAuthorSeung Hwan Park-
dc.contributor.alternativeName정경화-
dc.contributor.alternativeName이강문-
dc.contributor.alternativeName김훈-
dc.contributor.alternativeName윤기홍-
dc.contributor.alternativeName박승환-
dc.contributor.alternativeName박무영-
dc.identifier.bibliographicCitationBiochemistry and Molecular Biology International, vol. 44, no. 2, pp. 283-292-
dc.identifier.doi10.1080/15216549800201302-
dc.subject.keywordClostridium thermocellum-
dc.subject.keywordxylanase-
dc.subject.keywordcloning-
dc.subject.keywordxynX-
dc.subject.keywordexoglucanase-
dc.subject.localClostridium thermocellum-
dc.subject.localclostridium thermocellum-
dc.subject.localXylanase-
dc.subject.localxylanase-
dc.subject.localCloning-
dc.subject.localclonning-
dc.subject.localcloning-
dc.subject.localxynX-
dc.subject.localexoglucanase-
dc.description.journalClassY-
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