High-level expression of human insulin-like growth factor II in Escherichia coli

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Title
High-level expression of human insulin-like growth factor II in Escherichia coli
Author(s)
Hae Jin Rhee; Young Ik Lee; Kyu-Hwan Yang
Bibliographic Citation
Journal of Biotechnology, vol. 13, pp. 293-304
Publication Year
1990
Abstract
A gene encoding mature human insulin-like growth factor II (IGF-II) was constructed from the modified IGF-II cDNA sequence and two double-stranded synthetic oligodeoxynucleotide linkers. It was fused to a truncated lacZ gene such that IGF-II was expressed as part of C-terminus of β-galactosidase. This fused lacZ'-IGF-II gene was under the control of tac promoter and we overproduced the β-galactosidase-IGF-II fusion protein in the Escherichia coli. The fusion protein formed inclusion bodies inside the cells. The fusion protein was purified from the isolated inclusion bodies and IGF-II protein was obtained from their fusion protein by CNBr cleavage. The released IGF-II was confirmed by its molecular weight as determined by SDS-PAGE and by its ability to bind anti-iGF antibody.
Keyword
Human insulin-like growth factor IIβ-GalactosidaseFusion proteinE. coli
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/0168-1656(90)90077-O
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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