Overproduction of human granulocyte-colony stimulating factor fused to the pelB signal peptide in Escherichia coli

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Title
Overproduction of human granulocyte-colony stimulating factor fused to the pelB signal peptide in Escherichia coli
Author(s)
Bong Hyun Chung; Mi Jin Sohn; Su Wan Oh; Ui Sun Park; Haryoung Poo; Beom Soo Kim; Mi Jin Yu; Young Ik Lee
Bibliographic Citation
Journal of Fermentation Bioengineering, vol. 85, no. 4, pp. 443-446
Publication Year
1998
Abstract
In an attempt to induce the secretion of human granulocyte-colony stimulating factor (hG-CSF) in Escherichia coli, the gene encoding mature hG- CSF was fused to the gene for the pectate lyase B (pelB) signal peptide of Erwinia caratovora, and fed-batch fermentation of the recombinant E. coli was performed. No secretion of hG-CSF and no processing of the signal peptide from the fusion protein was observed. Instead, the fusion protein, pelB-hG- CSF, at a concentration of ca. 1.7 g/l was produced in an inclusion body form. Interestingly, the biological activity of the purified fusion protein was found to be identical to that of mature hG-CSF, indicating that the pelB signal peptide attached to the N-terminal of hG-CSF had no effect on its biological activity.
Keyword
Escherichia coliHuman granulocyte-colony stimulating factor
ISSN
0385-6380
Publisher
Soc Bioscience Bioengineering Japan
DOI
http://dx.doi.org/10.1016/S0922-338X(98)80092-5
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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