DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hye Kyung Chung | - |
dc.contributor.author | Seong Hun Lee | - |
dc.contributor.author | Hyung Hoan Lee | - |
dc.contributor.author | Dae Sil Lee | - |
dc.contributor.author | Yong Sung Kim | - |
dc.date.accessioned | 2017-04-19T08:55:20Z | - |
dc.date.available | 2017-04-19T08:55:20Z | - |
dc.date.issued | 1994 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4397 | - |
dc.description.abstract | The cDNA of the infectious pancreatic necrosis virus (IPNV) DRT strain was synthesized using the reverse transcriptase (RT)-polymerase chain reaction (PCR) and its cDNA nucleotide sequence was determined. The cDNA nucleotide sequence of the segment A was 3,155 base pairs (bp) in length and con tained two overlapping open reading frames (ORFs). The large ORF was 2,916 bp and predicted a polyprotein of a molecular weight of 106,641 daltons, whereas the small ORF was a 444 bp and predicted a protein of a molecular weight of 17,369 in lPNV-DRT. The large ORF contains three genes, VP2-NS-VP3, which make a polyprotein. lPNV-ORT and lPNV-Jasper polyproteins are closely related (99% amino acid homology). Most of the amino acid mismatches are in VP2 sequences, mainly within the area of the serotype-specific epitope. The small ORF overlapped with the N-terminal part of polyprotein. The 3' terminal sequence of the ORT strain is longer by 58 bp and 51 bp, respectively, than those of the Jasper and N 1 strains. The ORT strain segment A sequence may not contain a tetranucleotide sequence (CCCC) at the 3' end. The nucleotide sequence data of the segment A in this paper has been deposited with the GSDB, DDBJ, EMBL and NCBI nucleotide sequence databases and has been assigned accession number 026526. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Nucleotide sequence analysis of the VP2-NS-VP3 genes of infectious pancreatic necrosis virus DRT strain | - |
dc.title.alternative | Nucleotide sequence analysis of the VP2-NS-VP3 genes of infectious pancreatic necrosis virus DRT strain | - |
dc.type | Article | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.number | 0 | - |
dc.citation.endPage | 354 | - |
dc.citation.startPage | 349 | - |
dc.citation.volume | 4 | - |
dc.contributor.affiliatedAuthor | Dae Sil Lee | - |
dc.contributor.affiliatedAuthor | Yong Sung Kim | - |
dc.contributor.alternativeName | 정혜경 | - |
dc.contributor.alternativeName | 이성훈 | - |
dc.contributor.alternativeName | 이형환 | - |
dc.contributor.alternativeName | 이대실 | - |
dc.contributor.alternativeName | 김용성 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, vol. 4, pp. 349-354 | - |
dc.description.journalClass | Y | - |
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