Biotic and abiotic stress-related expression of 1-aminocyclopropane-1-carboxylate oxidase gene family in Nicotiana glutinosa L.

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Title
Biotic and abiotic stress-related expression of 1-aminocyclopropane-1-carboxylate oxidase gene family in Nicotiana glutinosa L.
Author(s)
Yeon Sup Kim; Do Il Choi; Myeong Min Lee; Sun Hi Lee; Woo Taek Kim
Bibliographic Citation
Plant and Cell Physiology, vol. 39, no. 6, pp. 565-573
Publication Year
1998
Abstract
Three full length 1-aminocyclopropane-1-carboxylate (ACC) oxidase cDNA clones (pNG-ACO1, 1,254 bp; pNGACO2, 1,198 bp; and pNG-ACO3, 1,053 bp) were isolated from the TMV-treated leaf cDNA library of Nicotiana glutinosa plant. They share a high degree of sequence identity (78-81%) throughout the coding regions but are divergent within the 3'-untranslated regions. The gene-specific probes were prepared using these regions to investigate the differential expression of the ACC oxidase gene family in various organs and in response to a multitude of biotic and abiotic stresses in N. glutinosa plants. All three genes were transcriptionally active displaying unique patterns of expression. Both the pNG-ACO1 and pNG-ACO3 transcripts highly accumulated during the senescence of leaves, while the pNG-ACO2 mRNA was constitutively present. In addition, the NG-ACO1 and NG-ACO3 transcripts were predominantly found in roots whereas the NG-ACO2 mRNA was mainly in stems. Upon TMV infection, both NGACO1 and NG-ACO3 were markedly induced, but in mock treatment which has an effect of mild wounding, only the NG-ACO3 gene was induced. Furthermore, salicylic acid and CuSO4 treatments of leaves increased the level of NGACO1 and NG-ACO3 transcripts, while they did not affect the NG-ACO2 gene expression. Results showed that both the NG-ACO1 and NG-ACO3 genes were highly inducible by ethylene and methyl jasmonate treatments, with NGACO3 being more responsive. By contrast, NG-ACO2 did not respond to these growth regulators. Thus, it appears that there are two groups of ACC oxidase transcripts expressed in leaf tissue of N. glutinosa, either stress-induced or constitutive. The possible molecular mechanism of differential regulation of ACC oxidase gene expression and its physiological significance are discussed.
Keyword
1-Aminocyclopropane-1-carboxylate (ACC) oxidaseDifferential expressionGene familyNicotiana glutinosaStress response
ISSN
0032-0781
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/oxfordjournals.pcp.a029406
Type
Article
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1. Journal Articles > Journal Articles
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