DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kyung Hwa Jung | - |
dc.contributor.author | Yong Chin Chun | - |
dc.contributor.author | Jae Chan Lee | - |
dc.contributor.author | Seung Hwan Park | - |
dc.contributor.author | Ki Hong Yoon | - |
dc.date.accessioned | 2017-04-19T08:55:22Z | - |
dc.date.available | 2017-04-19T08:55:22Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/4411 | - |
dc.description.abstract | β-Xylosidase was purified from the recombinant Escherichia coli carrying the Bacillus sp. KK-1 β-xylosidase gene (xylB). The molecular mass of the purified enzyme was estimated to be 62 kDa by sodium dodecyl sulfate- polyacrylamide gel electrophoresis. However, the apparent molecular mass of the β-xylosidase was 140 kDa, indicating that the native β-xylosidase has an oligomeric structure composed of two identical subunits. The isoelectric point was determined to be pH 5.5. The enzyme was highly active on p- nitrophenyl-β-D-xylopyranoside but it barely hydrolyzed xylan substrates, and did not exhibit activity towards carboxymethylcellulose and p- nitrophenyl-β-D-glucopyranoside. The enzyme had a pH optimum for its activity at pH 6.5 and a temperature optimum at 40°C. The enzyme activity was completely inhibited by the presence of Hg++, and also markedly inhibited by D-xylose and D-glucose. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Purification and characterization of the Bacillus sp. KK-1 β-xylosidase from a recombinant Escherichia coli | - |
dc.title.alternative | Purification and characterization of the Bacillus sp. KK-1 β-xylosidase from a recombinant Escherichia coli | - |
dc.type | Article | - |
dc.citation.title | Journal of Microbiology and Biotechnology | - |
dc.citation.number | 3 | - |
dc.citation.endPage | 263 | - |
dc.citation.startPage | 258 | - |
dc.citation.volume | 8 | - |
dc.contributor.affiliatedAuthor | Kyung Hwa Jung | - |
dc.contributor.affiliatedAuthor | Seung Hwan Park | - |
dc.contributor.alternativeName | 정경화 | - |
dc.contributor.alternativeName | 전용진 | - |
dc.contributor.alternativeName | 이재찬 | - |
dc.contributor.alternativeName | 박승환 | - |
dc.contributor.alternativeName | 윤기홍 | - |
dc.identifier.bibliographicCitation | Journal of Microbiology and Biotechnology, vol. 8, no. 3, pp. 258-263 | - |
dc.subject.keyword | Bacillus | - |
dc.subject.keyword | β-xylosidase | - |
dc.subject.keyword | purification | - |
dc.subject.local | Bacillus | - |
dc.subject.local | bacillus | - |
dc.subject.local | β-xylosidase | - |
dc.subject.local | Purification | - |
dc.subject.local | purification | - |
dc.subject.local | Purifcation | - |
dc.description.journalClass | Y | - |
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