A biotin-avidin labeled enzyme immunoassay for the quantitation of serum TSH using protein-layered solid phase

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dc.contributor.authorMyung Ja Choi-
dc.contributor.authorEun Young Song-
dc.contributor.authorTai Wha Chung-
dc.date.accessioned2017-04-19T08:55:23Z-
dc.date.available2017-04-19T08:55:23Z-
dc.date.issued1998-
dc.identifier.issn0253-6269-
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/4414-
dc.description.abstractA sensitive enzyme immunoassay for serum TSH has been developed utilizing the tight binding between biotin and avidin, and three layered protein polystyrene beads as solid phase. To increase binding capacity of TSH and sensitivity of the assay, the polystyrene beads were coated sequentially with mouse immunoglobulin as first layer, rabbit antimouse immunoglobulin as second layer and monoclonal anti-TSH as third layer. A serum sample was incubated simultaneously with a monoclonal anti-TSH immobilized polystyrene beads and a second monoclonal anti-TSH covalently attached to biotin. After washing, the antibody bound serum TSH-anti-TSH-biotin complex is reacted with horseradish peroxidase (HRP)-labeled avidin. Following a second wash, the bound HRP activity was measured colorimetrically. Reproducible results were obtained within 4 hours for serum TSH in the range between 0 μlU/ml and 50 μlU/ml with detection limit of 0.1 μlU per test.-
dc.publisherPharmaceutical Soc Korea-
dc.titleA biotin-avidin labeled enzyme immunoassay for the quantitation of serum TSH using protein-layered solid phase-
dc.title.alternativeA biotin-avidin labeled enzyme immunoassay for the quantitation of serum TSH using protein-layered solid phase-
dc.typeArticle-
dc.citation.titleArchives of Pharmacal Research-
dc.citation.number3-
dc.citation.endPage235-
dc.citation.startPage231-
dc.citation.volume21-
dc.contributor.affiliatedAuthorEun Young Song-
dc.contributor.affiliatedAuthorTai Wha Chung-
dc.contributor.alternativeName최명자-
dc.contributor.alternativeName송은영-
dc.contributor.alternativeName정태화-
dc.identifier.bibliographicCitationArchives of Pharmacal Research, vol. 21, no. 3, pp. 231-235-
dc.identifier.doi10.1007/BF02975280-
dc.subject.keywordBiotin-avidin-
dc.subject.keywordEIA-
dc.subject.keywordTrilayered solid phase-
dc.subject.keywordTSH-
dc.subject.localBiotin-avidin-
dc.subject.localEIA-
dc.subject.localTrilayered solid phase-
dc.subject.localTSH-
dc.description.journalClassY-
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